PLC_1 (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70616m |
| Product Name | PLC_1 (N-terminal region) Antibody |
| Applications |
WB
IP
|
| Specificity | The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain. This sequence is highly conserved in rat and mouse PLCγ1, and has low homology to PLCγ2. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCγ1. |
| Clonality | Monoclonal |
| Clone # | M156 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P19174 |
| Synonyms | Phospholipase C gamma1, phosphodiesterase |
| Background | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLCδ is activated by both receptor and nonreceptor tyrosine kinases. PLCγ1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLCγ1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity. |
| Application Dilution | |
| WB | |
| IP | |