| Overview |
| bsm-70616m |
| PLC_1 (N-terminal region) Antibody |
| WB, IP |
| The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain. This sequence is highly conserved in rat and mouse PLC_1, and has low homology to PLC_2. |
| Human, Mouse, Rat, Chicken |
| Specifications |
| Unconjugated |
| Mouse |
| Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLC_1. |
| Monoclonal |
| M156 |
| IgG1 |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| P19174 |
| Phospholipase C gamma1, phosphodiesterase |
| Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLCs have been identified: PLC_, PLC_, PLC_, and PLC_. Phosphorylation is one of the key mechanisms that regulates the activity of PLC. PLC_ is activated by both receptor and nonreceptor tyrosine kinases. PLC_1 forms a complex with EGF and PDGF receptors, which leads to phosphorylation at tyrosine 771, 783, and 1245. In addition, antigen receptor-induced activation of PLC_1 leads to phosphorylation at both Tyr-775 and Tyr-783. These two sites are equally important for activation of enzymatic activity. |
| Application Dilution |
| WB |
|
| IP |
|
Powered by Bioz