| Overview |
| bsm-70687m |
| Stat5 (C-terminal region) Antibody |
| WB |
| The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells. This sequence has high homology to the conserved regions in rat and mouse Stat5A and B. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| Clone (M251) was generated from a recombinant protein that included amino acid residues in the C-terminal region of human Stat5A. |
| Monoclonal |
| M251 |
| IgG1 |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| P42229 |
| Signal transducer and activator of transcription 5A, Stat5A |
| The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFN_ in B cells, but only Stat5A is phosphorylated in response to IFN_ in HeLa cells. |
| Application Dilution |
| WB |
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