Stat5 (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70687m |
| Product Name | Stat5 (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells. This sequence has high homology to the conserved regions in rat and mouse Stat5A and B. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M251) was generated from a recombinant protein that included amino acid residues in the C-terminal region of human Stat5A. |
| Clonality | Monoclonal |
| Clone # | M251 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P42229 |
| Synonyms | Signal transducer and activator of transcription 5A, Stat5A |
| Background | The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells. |
| Application Dilution | |
| WB | |