Synapsin I (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70690M-100uL |
| Product Name | Synapsin I (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects an 80 kDa* protein corresponding to the molecular mass of Synapsin I on SDS-PAGE immunoblots of adult mouse brain. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M365 was generated from a sequence corresponding to amino acids in the C-terminal region of mouse synapsin I. This sequence has high homology to human and rat synpasin I. |
| Clonality | Monoclonal |
| Clone # | M365 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | O88935 |
| Synonyms | SYN1, SYN1a, SYN1b, SynapsinI, SYNI |
| Background | Synapsins are important peripheral membrane proteins involved in synaptic vesicle release during neurotransmission between neurons. Several synapsin variants are generated by alternative splicing of the mRNA from three different genes: synapsin I, II, and III. Synapsins bind to synaptic vesicles via their conserved N-terminal domains. Regulation of synapsins may occur through PKA and CAM kinase I phosphorylation. This regulation occurs concomitantly with membrane excitation and neurotransmitter release into the synaptic cleft. Synapsins bundle actin filaments and interact with multiple calcium binding proteins. Thus, synapsins may crosslink vesicles or other proteins to actin filaments at the presynaptic terminal. In addition, synapsins may interact with each other, since synapsin III has been shown to form heteromultimers with both synapsin I and synapsin II. All synapsins contain a central ATP binding domain and variable C-terminal domains that facilitate distinct regulatory functions. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Synapsin I (C-terminal region) Antibody (bsm-70690M) validated for, and at what dilution?
bsm-70690M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Synapsin I (C-terminal region) Antibody (bsm-70690M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70690M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Synapsin I (C-terminal region) Antibody (bsm-70690M)?
Clone M365 was generated from a sequence corresponding to amino acids in the C-terminal region of mouse synapsin I. This sequence has high homology to human and rat synpasin I.