Trop-2/TACSTD2 (Extracellular region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70695M-100uL |
| Product Name | Trop-2/TACSTD2 (Extracellular region) Antibody |
| Applications |
WB
IP
|
| Specificity | Clone M005 detects a 50-65 kDa* band corresponding to the molecular mass of Trop-2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured Trop-2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line. |
| Clonality | Monoclonal |
| Clone # | M005 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P09758 |
| Synonyms | Tumor-associated calcium signal transducer 2; Trop-2; Membrane component chromosome 1 surface marker 1; GA733-1; TACSTD2; M1S1; TROP2 |
| Background | Trop-2 (TACSTD2) is a transmembrane glycoprotein found on invasive trophoblast cells and in several epithelial type cancer cells. Trop-2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site. This glycoprotein functions in many signaling pathways including interaction of its extracellular domain with integrin β1 to regulate FAK signaling, interaction of its transmembrane domain with claudin 1 and claudin 7 during tight junction formation, and regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway. These Trop-2 functions may be important during tumor proliferation, metastasis and invasion. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Trop-2/TACSTD2 (Extracellular region) Antibody (bsm-70695M) validated for, and at what dilution?
bsm-70695M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Trop-2/TACSTD2 (Extracellular region) Antibody (bsm-70695M) react with?
Tested reactivity: Human.
How should bsm-70695M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Trop-2/TACSTD2 (Extracellular region) Antibody (bsm-70695M)?
Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line.