Phospho-Paxillin (Y118) Recombinant Antbody Kit (KD-Validated)

$Immunocytochemical staining of Hela cells with anti-Phospho-Paxillin (Y118) antibody (1:1,000). Nuclei were stained blue with DAPI; Phospho-Paxillin (Y118) was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.$

Immunocytochemical staining of Hela cells with anti-Phospho-Paxillin (Y118) antibody (1:1,000). Nuclei were stained blue with DAPI; Phospho-Paxillin (Y118) was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.

$Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.$

Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.

$Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Phospho-Paxillin (Y118) expression in wild type (WT) and Phospho-Paxillin (Y118) shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulinserves as a loading control. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.$

Western blotting analysis using anti-Phospho-Paxillin (Y118) antibody. Phospho-Paxillin (Y118) expression in wild type (WT) and Phospho-Paxillin (Y118) shRNA knockdown (KD) HeLa cells with 20 \u03bcg of total cell lysates . \u03b2-Tubulinserves as a loading control. The blot was incubated with anti-Phospho-Paxillin (Y118) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ\u2122 ECL Substrate Kit.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-90447r-50µL-ab-25ug-cell-lysate-25ug-wt-cell
Product Name	Phospho-Paxillin (Y118) Recombinant Antbody Kit (KD-Validated)
Applications	WB, IC
Specificity	Knockdown Validated
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human Paxillin
Immunogen Range	100-165/165
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Affinity Purified
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	-20 °C
Target
Gene ID	5829
Swiss Prot	P49023
Background	Cell line: Hela Knockdown technology: shRNA Knockdown
Application Dilution
WB	1:300-5000
IC	1:1000