Peptidylprolyl Isomerase A Recombinant Antbody Kit (KD-Validated)

Flow cytometric analysis of Peptidylprolyl isomerase A expression in HepG2 cells using anti-Peptidylprolyl isomerase A antibody (1:2,000). Green, isotype control; red, Peptidylprolyl isomerase A.

$Immunocytochemical staining of HepG2 cells with anti-Peptidylprolyl isomerase A antibody (1:1,000). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase A was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.$

Immunocytochemical staining of HepG2 cells with anti-Peptidylprolyl isomerase A antibody (1:1,000). Nuclei were stained blue with DAPI; Peptidylprolyl isomerase A was stained magenta with Alexa Fluor\u00ae 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 \u03bcm.

$Western blotting analysis using anti-Peptidylprolyl isomerase A antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.$

Western blotting analysis using anti-Peptidylprolyl isomerase A antibody. Total cell lysates (30 \u03bcg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Peptidylprolyl isomerase A antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ\u2122 ECL Substrate Kit.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-90448r-50µL-ab-25ug-cell-lysate-25ug-wt-cell
Product Name	Peptidylprolyl Isomerase A Recombinant Antbody Kit (KD-Validated)
Applications	WB, FCM, IC
Specificity	Knockdown Validated
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human Cyclophilin A
Immunogen Range	180-224/224
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Affinity Purified
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	-20 °C
Target
Gene ID	5478
Swiss Prot	P62937
Background	Cell line: HeLa Knockdown technology: shRNA Knockdown
Application Dilution
WB	1:300-5000
FCM	1:20-100
IC	1:1000