Mouse LRP1 Ready-To-Use IHC Kit
Mouse LRP1 Ready-To-Use IHC Kit
Cat. No.: IHC0569M
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
CD91 is a 600 kDa type I transmembrane protein composed of an alpha and beta chain. The alpha chain
interacts with a variety of structurally and functionally diverse ligands, while the beta chain contains two tyrosine
phosphorylation motifs that interact with adaptor and signaling proteins. This receptor is expressed by cells in
the monocytic lineage, dendritic cells, hepatocytes, fibroblasts, adipocytes, neurons, and syncytiotrophoblasts.
CD91 plays a crucial role in several cellular processes, including ligand endocytosis and trafficking to
lysosomes, intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. It is involved in cross-
presenting antigens via MHC class I molecules on antigen-presenting cells after endocytosis of heat shock
protein-peptide complexes. Additionally, CD91 is essential for the A2M-mediated clearance of secreted amyloid
precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients.
Expression of CD91 decreases with age and is found to be lower in brain tissue from Alzheimer patients
compared to controls. The receptor also plays a role in cell migration and activating signaling cascades,
highlighting its importance in both immune function and disease processes.
Synonyms
A2MR; AI316852; APOER; APR; b2b1554Clo; CD91; IGFBP3R; LRP; LRP1; LRP1A; TGFBR5; KPA; LDL receptor related protein 1.
Materials Supplied
| Number | Component | 50T | Concentration | Storage |
|---|---|---|---|---|
| 1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
| 2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
| 3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
| 4 | Blocking Buffer | 3ml | RTU | 2-8°C |
| 5 | Primary Antibody ((Mouse LRP1 Recombinant Rabbit mAb) | 6ml | RTU | 2-8°C |
| 6 | Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated) | 6ml | RTU | 2-8°C |
| 7 | Chromogen Component A | 0.3ml | RTU | -20°C |
| 8 | Chromogen Component B | 0.3ml | RTU | -20°C |
| 9 | Counter Staining Reagent | 5ml | RTU | RT |
| 10 | Differentiation Reagent | 6ml | RTU | RT |
| 11 | Mounting Media | 5ml | RTU | RT |
| 12 | Control slide (Mouse testis) | 1 slide | RTU | RT |
| 13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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