Human Osteopontin Ready-To-Use IHC Kit
Human Osteopontin Ready-To-Use IHC Kit
Cat. No.: IHC0343HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases close to the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and provide a novel substrate for plasmin and cathepsin D.
Synonyms
BNSP, BSPI, ETA-1, OPN, PSEC0156, SPP1, Bone sialoprotein 1, BSP I, Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, osteopontin, Secreted phosphoprotein 1, SPP 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin.Validation Data
Materials Supplied
| Number | Component | 50T | Concentration | Storage |
|---|---|---|---|---|
| 1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
| 2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
| 3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
| 4 | Blocking Buffer | 3ml | RTU | 2-8°C |
| 5 | Primary Antibody (Human Osteopontin Recombinant Rabbit mAb) | 6ml | RTU | 2-8°C |
| 6 | Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated) | 6ml | RTU | 2-8°C |
| 7 | Chromogen Component A | 0.3ml | RTU | -20°C |
| 8 | Chromogen Component B | 0.3ml | RTU | -20°C |
| 9 | Counter Staining Reagent | 5ml | RTU | RT |
| 10 | Differentiation Reagent | 6ml | RTU | RT |
| 11 | Mounting Media | 5ml | RTU | RT |
| 12 | Control slide (Human kidney) | 1 slide | RTU | RT |
| 13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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