Human ATP5A Ready-To-Use IHC Kit

Cat. No.: IHC0418H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoformshave been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Synonyms

AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; HEL-S-123m; MC5DN4; MOM2; ORM ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit 1; ATP synthase; H+ transporting; mitochondrial F1 complex; alpha subunit; isoform1; cardiac muscle; ATP synthase; H+ transporting; mitochondrial F1 complex;alpha subunit; isoform2; non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; ATP5AL2; ATP5AMOM2; ATPMATP synthase subunit alpha; mitochondrial; cardiac muscle;EC 3.6.3; EC 3.6.3.14; hATP1; mitochondrial ATP synthetase; oligomycin-resistant; OMR; ORMATP synthase alpha chain; mitochondrial.

Materials Supplied

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Number	Component	50T	Concentration	Storage
1	PBS Buffer (powder)	2L x 2	20x	RT
2	Antigen Retrieval Buffer	20ml	100x	2-8°C
3	Endogenous Peroxidase Blocking Buffer	3ml	RTU	2-8°C
4	Blocking Buffer	3ml	RTU	2-8°C
5	Primary Antibody (Human ATP5A Recombinant Rabbit mAb)	6ml	RTU	2-8°C
6	Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated)	6ml	RTU	2-8°C
7	Chromogen Component A	0.3ml	RTU	-20°C
8	Chromogen Component B	0.3ml	RTU	-20°C
9	Counter Staining Reagent	5ml	RTU	RT
10	Differentiation Reagent	6ml	RTU	RT
11	Mounting Media	5ml	RTU	RT
12	Control slide (human liver)	1 slide	RTU	RT
13	Datasheet	1 copy

Notes

1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
2. Do not allow slides to dry at any time during this procedure.
3. Please don't replace the matching reagents in this product with other manufacturers' products.
4. As DAB is a carcinogen, please take necessary precautions.
5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.