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Vimentin (Ser55) Polyclonal Antibody

HT1080 cell lysates probed with phospho-Vimentin (Ser55) Polyclonal Antibody, Unconjugated (bs-12757R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
HT1080 cell lysates probed with phospho-Vimentin (Ser55) Polyclonal Antibody, Unconjugated (bs-12757R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vimentin (Ser55) Polyclonal Antibody(bs-12757R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Vimentin (Ser55) Polyclonal Antibody(bs-12757R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U-87MG cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-12757R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human Hela cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U-87MG cell lysates probed with Vimentin Polyclonal Antibody, Unconjugated (bs-12757R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Horse
Overview
Catalog # bs-12757r-100uL
Product Name Vimentin (Ser55) Polyclonal Antibody
Applications
WB ELISA FCM
Reactivity Human
Predicted Reactivity Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser55
Modification Site Ser55
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7431
Swiss Prot P08670
Subcellular location Cytoplasm
Synonyms Vimentin (phospho S55); p-Vimentin (phospho S55); CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME_HUMAN; vimentin.
Background This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100

Frequently asked questions

Which applications is Vimentin (Ser55) Polyclonal Antibody (bs-12757R) validated for, and at what dilution?

bs-12757R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Vimentin (Ser55) Polyclonal Antibody (bs-12757R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Horse.

How should bs-12757R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Vimentin (Ser55) Polyclonal Antibody (bs-12757R)?

KLH conjugated synthesised phosphopeptide derived from human Vimentin around the phosphorylation site of Ser55

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