LOADING CONTROL SELECTION FOR DIFFERENT SAMPLES
Commonly used loading controls for proteins from the cytoplasm, mitochondria, and nucleus are discussed.
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Browse research updates, product guides, grant news, and practical antibody workflow articles from Bioss.
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How to select loading controls for Western Blots?
Considerations for Loading Control Selection
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Why do many researchers prefer polyclonal antibodies for an IHC/IF assay?
Rather than monoclonal antibodies, experienced scientists prefer to use polyclonal antibodies in IHC/IF assays
Read moreTips for Reducing Non-specific Staining in IHC
What can you do when non-specific staining in your IHC is obscuring your results and incurring your PI’s disdain?
Read moreImpacts of Polyacrylamide Concentration on Protein Experiments
Polyacrylamide is a common reagent in the molecular biology lab as it is used in the formation of gels used to study proteins in techniques such as Western blotting and polyacrylamide gel electrophoresis (PAGE).
Read more5 Tips for Reducing Non-specific Signal on Western Blots
Non-specific bands and high background on westerns can be a major source of frustration at the bench. The tips below are designed to help clean up background on your blots.
Read moreIntroduction to Recombinant Rabbit Monoclonal Antibodies
Via the utilization and adaption of emerging technologies, Bioss has engineered recombinant rabbit monoclonal antibodies for greater specificity and ideal target detection. Read about our process and the advantages this highlighted product line.
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Antibody Manufacturing: The Power of Small Peptides
Through a proprietary process, Bioss engineers antibodies using short peptide antigens for greater selectivity and ideal detection. Read about our process and the advantages over native protein antigens.
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Our Comprehensive Guide to Performing Double Immunofluorescence
Learn the process of double staining for immunofluorescence and the advantages and disadvantages of using different antibodies for the procedure
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Fluorescent vs. Chromogenic Detection in Immunohistochemistry
Learn the differences between fluorescent and chromogenic detection techniques for analyzing immunohistochemistry (IHC) experiments
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How current, voltage, and power settings affect SDS-PAGE
A general outline of the electrical principles of SDS-PAGE and considerations for running Western blots using a constant current or constant voltage powerpack
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Learn Western Blotting with These Open-Access Articles
We've compiled a brief list of some of the best open-access journal articles for learning the basic theory and practice of Western Blotting
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