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Catalog #1: bs-5397R-BF594

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-5397R-BF555

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-5397R-BF488

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-5397R-BF405

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-5397R-BF350

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-5397R-PE

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-5397R-FITC

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-5397R-Cy7

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-5397R-Cy5.5

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-5397R-Cy5

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-5397R-Cy3

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Cy3

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS-2(Ser731)) polyclonal Antibody, Unconjugated (bs-5397R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS-2(Ser731)) polyclonal Antibody, Unconjugated (bs-5397R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
SH-SY5Y cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-IRS-2(Ser731) Polyclonal Antibody(bs-5397R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-5397R

Applications: FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1:Rat Cerebrum lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human HepG2 cell lysates probed with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Testis tissue lysates probed with LC3B Polyclonal Antibody, Unconjugated (bs-4843R) at dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4843R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6655R-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: RBITC

Catalog #1: bs-6655R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy5.5

Catalog #1: bs-6655R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP

Catalog #1: bs-6655R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy7

Catalog #1: bs-6655R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5.5

Catalog #1: bs-6655R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5

Catalog #1: bs-6655R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy7

Catalog #1: bs-6655R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5.5

Catalog #1: bs-6655R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC

Catalog #1: bs-6655R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-750

Catalog #1: bs-6655R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-680

Catalog #1: bs-6655R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-647

Catalog #1: bs-6655R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-594

Catalog #1: bs-6655R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-555

Catalog #1: bs-6655R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-488

Catalog #1: bs-6655R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-405

Catalog #1: bs-6655R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor-350

Catalog #1: bs-6655R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-6655R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-6655R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-6655R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-6655R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-6655R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Lane 1: mouse lung lysates Lane 2: human colon carcinoma lysates probed with Anti Cryopyrin\/CIAS1\/NALP3 Polyclonal Antibody, Unconjugated (bs-6655R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 114kD. Observed band size: 40kD, 114kD.
RAW264.7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with NALP3\/CIAS1 Antibody(bs-6655R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-6655R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-20222R-RBITC

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-20222R-PerCP-Cy5.5

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-20222R-PerCP

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-20222R-PE-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-20222R-PE-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-20222R-PE-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-20222R-APC-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-20222R-APC-Cy5.5

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-20222R-APC

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-20222R-BF750

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-20222R-BF680

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680