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Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha\/Beta\/Gamma\/Delta\/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 31kD. Observed band size: 31kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0237r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with 14-3-3 beta Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with 14-3-3 beta Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-12420R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12420R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-12420R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-12420R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-12420R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-12420R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-12420R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-12420R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-12420R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-12420R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-12420R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-12420R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-12420R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-12420R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-12420R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-12420R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-12420R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-12420R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-12420R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-12420R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-2340R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2340R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-2340R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-2340R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-2340R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-2340R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-2340R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-2340R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-2340R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-2340R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-2340R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bs-2340R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-2340R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2340R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2340R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-2340R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2340R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2340R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-2340R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2340R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Oat lysates probed with 14-3-3 family protein Polyclonal Antibody, Unconjugated (bs-2017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Wheat lysates probed with 14-3-3 family protein Polyclonal Antibody, Unconjugated (bs-2017R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-2017r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-2017R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-350

Catalog #1: bs-2017R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-405

Catalog #1: bs-2017R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-488

Catalog #1: bs-2017R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-555

Catalog #1: bs-2017R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-594

Catalog #1: bs-2017R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: AbBy Fluor-647