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Catalog #1: bsm-52004r-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52004r-pe-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52004r-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-52004r-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-52004r-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-52004r-percp-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52004r-percp-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52004r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-52004r-rbitc-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-60374m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52728r

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 sigma Monoclonal Antibody, Unconjugated (bsm-52005R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52005r

Applications: IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52005r-bf350-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-52005r-bf405-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52005r-bf488-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52005r-bf555-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52005r-bf594-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52005r-bf647-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52005r-bf680-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52005r-bf750-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52005r-apc-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-52005r-apc-Cy5.5-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-52005r-biotin-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-52005r-cy3-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-52005r-cy5-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-52005r-Cy5.5-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-52005r-cy7-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-52005r-fitc-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-52005r-hrp-100ul

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-52005r-pe-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-52005r-percp-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-52005r-percp-Cy5.5-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52005r-rbitc-100ul

Applications: IF

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 28 kDa\r\nObserved MW: 28 kDa\r\n
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M
Tissue: esophagus\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M

Catalog #1: bsm-60449m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52006r

Applications: WB, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: mouse brain lysates; Lane 5: C6 Cell lysates; Lane 6: PC-12 Cell lysates; probed with YWHAZ (1B6) Monoclonal Antibody, unconjugated (bsm-51397M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YWHAZ (1B6) Monoclonal Antibody, Unconjugated (bsm-51397M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51397M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: NIH-3T3, 3: BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa

Catalog #1: bsm-60209R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-52008m

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with 5 Lipoxygenase monoclonal antibody, unconjugated (bsm-61061R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated(bsm-61061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated(bsm-61061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated(bsm-61061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-61061r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61061r-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61061r-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61061r-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61061r-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61061r-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61061r-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61061r-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61061r-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61061r-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC