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Catalog #1: bs-20782R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-20781R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43032r

Applications: IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Tissue: Human spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60605R
Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60605R
Cell line: Raji\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60605R
Cell line: Raji\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 90% Methanol\r\nPrimary ab dilution: 1:100\r\nSecondary ab: Goat anti Rabbit IgG\r\nUnlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).\r\nIsotype control: Rabbit monoclonal IgG (Blue line).\r\nComment: Line red is the positive signal for bsm-60605R

Catalog #1: bsm-60605r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-60900R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-60900R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-60900R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD19 Monoclonal Antibody, Unconjugated(bsm-60900R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60900r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti CD1a\/Cortical thymocyte antigen CD1A Polyclonal Antibody, Unconjugated (bs-3786R) at 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti CD1a\/Cortical thymocyte antigen CD1A Polyclonal Antibody, Unconjugated (bs-3786R) at 1:300 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3786R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Molt-4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD1a Polyclonal Antibody,Unconjugated (bs-22573R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human Jurkat cell lysates probed with CD1a Polyclonal Antibody, Unconjugated (bs-22573R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-22573R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human Thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD1A ) Monoclonal Antibody, Unconjugated (bsm-60907R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60907r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60395M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: THP-1 (Negative control), 2: MOLT-4, 3: Jurkat Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 38 kDa Observed MW: 43 kDa
Tissue: Human tonsil Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R
Tissue: Human spleen Section type: Formalin-fixed &\r\nParaffin-embedded section Retrieval method: High temperature and high\r\npressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60660R

Catalog #1: bsm-60660r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60537m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Rat brain and heart lysates probed with CD1d1 Polyclonal Antibody, unconjugated (bs-3690R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:3000 for 90 minutes at 37\u00b0C.\\n
Mouse spleen cells probed with \\tCD1d1 Polyclonal Antibody, Unconjugated (bs-3690R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-3690R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse liver lysates Lane 2: mouse thymus lysates probed with Anti CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Observed band size: 34kD
Mouse splenocytes probed with CD1d1 Polyclonal Antibody, Unconjugated (bs-4961R) at 1:100 for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-4961R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-23497R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60411M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD2 monoclonal antibody, unconjugated (bsm-63073R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Molt-4 (H) cell; Antibody incubation with (CD2) monoclonal Antibody, unconjugated (bsm-63073R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD2 antibody (bsm-63073R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-63073r-100ul

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-100510c-100ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD2 Monoclonal Antibody, Unconjugated(bsm-60313R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60313r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD2) Monoclonal Antibody, Unconjugated (bsm-33075M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33075m

Applications: FCM

Reactivity:

Conjugate: Unconjugated

Raji cell lysates probed with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52346R

Applications: WB, IHC-P, IHC

Reactivity: Human, Monkey

Conjugate: Unconjugated

Catalog #1: bsm-51506m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD20 monoclonal antibody, unconjugated (bsm-33054M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD20 Monoclonal Antibody, Unconjugated (bsm-33054M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33054m-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD20 Polyclonal Antibody (bs-0080R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human larynocarcinoma labeled with Anti-CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0080r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse lymph node lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-20639R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Mouse thymus lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-20639R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.

Catalog #1: bs-20639R

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-25072R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25660r

Applications: ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human Raji cell lysates probed with CD20 Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60638r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD20 antibody (bsm-63327R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with CD20 monoclonal antibody, unconjugated (bsm-63327R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD20 Monoclonal Antibody, Unconjugated(bsm-63327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD20 Monoclonal Antibody, Unconjugated(bsm-63327R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63327r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD200\/MOX1 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD200\/MOX1 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human Colon Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200 Polyclonal Antibody, Unconjugated (bs-6030R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.

Catalog #1: bs-6030R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-10521R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Staining of Placental villus from 7w gestation karyotype-normal pregnancy with bs-7351R. The Positive Control used is Placental villus from 7w gestation karyotype-normal pregnancy. The Negative Control used was KLH antibody. Images were taken at 400x magnification, bars represent 60\u00b5m. Figure legend: VC: villus core, CT: cytotrophoblast, ST: syncytiotrophoblast, MB: maternal blood space bathing placental villi. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-60546R

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD206) Monoclonal Antibody, Unconjugated (bsm-60761R) at 1:300 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60761r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human SKin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD207 Monoclonal Antibody, Unconjugated(bsm-61646R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD207 Monoclonal Antibody, Unconjugated(bsm-61646R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61646r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2239R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61560r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Cell line: Raji <br>\r\nFixative: 4% Paraformaldehyde\t<br>\r\nPermeabilization: 0.1% TritonX-100\t<br>\r\nPrimary ab dilution: 1:100\t<br>\r\nPrimary incubation condition: 1 hour at room temperature\t<br>\r\nSecondary ab: Goat Anti-Rabbit IgG\t<br>\r\nNuclear counter stain: DAPI (Blue)\t<br>\r\nComment: Color green is the positive signal for bsm-60246R\t<br>
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD21 Monoclonal Antibody, Unconjugated(bsm-60246R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD21 Monoclonal Antibody, Unconjugated(bsm-60246R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD21 Monoclonal Antibody, Unconjugated(bsm-60246R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60246r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1481R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Cell line: Daudi<br>\r\nFixative: 4% Paraformaldehyde\t<br>\r\nPermeabilization: 0.1% TritonX-100\t<br>\r\nPrimary ab dilution: 1:100\t<br>\r\nPrimary incubation condition: 1 hour at room temperature\t<br>\r\nSecondary ab: Goat Anti-Rabbit IgG\t<br>\r\nNuclear counter stain: DAPI (Blue)\t<br>\r\nComment: Color green is the positive signal for bsm-60233R\t<br>
\tParaformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD22 Monoclonal Antibody, Unconjugated(bsm-60233R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with CD22 monoclonal antibody, unconjugated (bsm-60233R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60233r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2529R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

The Daudi(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-CD23 antibody (bsm-33088M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC (bs-60296G-FITC): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-33088M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33088m-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse spleen lysates probed with CD23 Polyclonal Antibody, Unconjugated (bs-2490R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: 293T cell lysates probed with CD23\/Fc EpsilonR 2 Polyclonal Antibody, Unconjugated (bs-2490R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2490R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD23) Monoclonal Antibody, Unconjugated (bsm-60453R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD23) Monoclonal Antibody, Unconjugated (bsm-60453R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Antibody incubation with CD23 Monoclonal Antibody, Unconjugated(bsm-60453R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD23 Monoclonal Antibody, Unconjugated (bsm-60453R) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-60453r-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60583m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7590R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD239 Monoclonal Antibody, Unconjugated (bsm-54684R) at 1:200 for 30 minutes at room temperature, DAB staining.
Flow cytometric analysis of CD239 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54684R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54684R

Applications: WB, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Human prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (bsm-60746R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (bsm-60746R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD239) Monoclonal Antibody, Unconjugated (bsm-60746R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Human TT cell lysates probed with CD239 Monoclonal Antibody, Unconjugated (bsm-60746R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60746r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

U-937 cells were stained with CD24 Polyclonal Antibody(bs-4891R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates probed with CD24 Polyclonal Antibody, Unconjugated (bs-4891R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4891R

Applications: WB, ELISA, FCM, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated