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Catalog #1: bs-42274R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60413m

Applications: Flow-Cyt, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-61034r-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60882r-100ul

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-54156R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.

Catalog #1: bsm-54156r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30073m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43685m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30073m-apc-100t

Applications: FCM

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-30073m-apc-cy7-100t

Applications: FCM

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-30073m-fitc-100t

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30073m-pe-100t

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30073m-pe-cy7-100t

Applications: FCM

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-30073m-percp-Cy5.5-100t

Applications: FCM

Reactivity: Human

Conjugate: PerCP-Cy5.5

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CD11b\/Integrin \u03b1M\/Integrin Alpha M Polyclonal Antibody, Unconjugated (bs-1014R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with CD11b\/c Polyclonal Antibody, Unconjugated (bs-1014R ) (0.2ug) for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control unstained cells (blue), secondary only (light blue) and isotype control (orange).
This image was kindly submitted by Kandy Velazquez from USC School of Medicine.\r\nMouse colon lysates probed with CD11b\/c Polyclonal Antibody, unconjugated (bs-1014R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Rat Spleen tissue lysates probed with CD11b Polyclonal Antibody, Unconjugated (bs-1014R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1014R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD11b monoclonal antibody, unconjugated (bsm-60737R) at 1:600 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-60737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-60737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11b Monoclonal Antibody, Unconjugated(bsm-60737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

Catalog #1: bsm-60737r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51510m

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (bsm-55624R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (bsm-55624R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (bsm-55624R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD11c Monoclonal Antibody, Unconjugated (bsm-55624R) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-55624r-100ul

Applications: WB, IHC-F, IF, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded: human esophageal cancer labeled with Rabbit Anti-CD11C Polyclonal Antibody, Unconjugated (bs-2508R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti Integrin Alpha X\/CD11c Polyclonal Antibody, Unconjugated (bs-2508R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti-Integrin Alpha X\/CD11c Polyclonal Antibody, Unconjugated (bs-2508R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-CD11b\/c Polyclonal Antibody, Unconjugated (bs-2508R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2508R

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-20689R

Applications: FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

THP-1 (H) cells were treated with or without PMA (50 ng\/mL) for 72 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with CD11c monoclonal antibody, unconjugated (bsm-61135R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD11c Monoclonal Antibody, Unconjugated(bsm-61135R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61135r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-CD13\/APN\/ANPEN Polyclonal Antibody, Unconjugated (bs-1383R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-CD13\/APN\/ANPEN Polyclonal Antibody, Unconjugated (bs-1383R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 probed with CD13\/APN\/ANPEN Polyclonal Antibody (bs-1383R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
U-937 (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CELF3 Antibody (bs-10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1383R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90070r-50uL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD13) Monoclonal Antibody, Unconjugated (bsm-60371R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD13) Monoclonal Antibody, Unconjugated (bsm-60371R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD13) Monoclonal Antibody, Unconjugated (bsm-60371R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD13) Monoclonal Antibody, Unconjugated (bsm-60371R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60371r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD13 monoclonal antibody, unconjugated (bsm-52618R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD13 Monoclonal Antibody, Unconjugated(bsm-52618R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD13 Monoclonal Antibody, Unconjugated(bsm-52618R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD13 Monoclonal Antibody, Unconjugated(bsm-52618R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52618r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-34036R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

LoVo lysates probed with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD133 Polyclonal Antibody, Unconjugated (bs-4770R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Lane 1: Mouse Sp2\/0 cell lysates; Lane 2: Human LOVO cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Mouse Uterus lysates probed with CD133 Polyclonal \r\nAntibody, Unconjugated (bs-4770R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4770R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0209R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with CD133 monoclonal antibody, unconjugated (bsm-62477R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (bsm-62477R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (bsm-62477R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD133 Monoclonal Antibody, Unconjugated (bsm-62477R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62477r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD134 Polyclonal Antibody, Unconjugated (bs-2685R) followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2685R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The MJ (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-CD134 antibody (bsm-60298R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60298r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD137\/TNFRSF9 Polyclonal Antibody, Unconjugated (bs-2449R) at 1:600 followed by conjugation to the secondary antibody
Lane 1: Mouse Thymus Lysates; Lane 2: Mouse Spleen Lysates; Lane 2: Mouse Lymph Lysates. Probed with CD137 polyclonal Antibody, unconjugated (bs-2449R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2449R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD138) Monoclonal Antibody, Unconjugated (bsm-60902R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD138) Monoclonal Antibody, Unconjugated (bsm-60902R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD138) Monoclonal Antibody, Unconjugated (bsm-60902R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD138) Monoclonal Antibody, Unconjugated (bsm-60902R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60902r-TR

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human Siha cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human HepG2 cell lysates probed with CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD14) Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-24777R

Applications: WB, ELISA

Reactivity: Mouse

Conjugate: Unconjugated

IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD14 (31G4) Monoclonal Antibody (bsm-52556R) at 1:100 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD14 (31G4) Monoclonal Antibody, Unconjugated (bsm-52556R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52556r

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-CD144\/VECD\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Images provided by the Independent Validation Program, badge number 029590:Formalin-fixed and frozen-OCT embedded mouse liver labeled with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated(bs-0878R) 1:100, overnight at 4\u00b0C, The secondary antibody was diluted to 1:100 for 120 minutes at 37\u00b0C.
A549 lyates probed with Rabbit Anti-VE Cadherin Polyclonal Antibody, Unconjugated (bs-0878R) at 1:300 in 4˚C overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-VE Cadherin Polyclonal Antibody (bs-0878R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0878R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HUVEC cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD144\/VE Cadherin Polyclonal Antibody(bs-22363R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human MCF-7 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates probed with CD144\/VE Cadherin Polyclonal Antibody, Unconjugated (bs-22363R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD144\/VE Cadherin) Polyclonal Antibody, Unconjugated (bs-22363R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-22363R

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61376r

Applications: WB, IP, ICC/IF

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-CD146\/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD146\/MCAM Polyclonal Antibody, Unconjugated (bs-1618R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Lane 1: U251 cell lysates; Lane 2: U-2os cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U2OS cell lysates; Lane 4: Human U251 cell lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-1618R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1618R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90119r-50uL

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD146 Monoclonal Antibody, Unconjugated(bsm-61136R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD146 Monoclonal Antibody, Unconjugated(bsm-61136R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD146 Monoclonal Antibody, Unconjugated(bsm-61136R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD146 Monoclonal Antibody, Unconjugated(bsm-61136R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61136r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with CD147 Polyclonal Antibody, unconjugated (bs-0684R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
MCF-7 cells probed with CD147 Polyclonal Antibody, Unconjugated (bs-0684R) at 3ug for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human MCF-7 cell lysates probed with CD147 Polyclonal Antibody, Unconjugated (bs-0684R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0684R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CD147 (12C3) Monoclonal Antibody (bsm-52837R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52837r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70409M

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70410M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60410M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-63326M) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63326m-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-60940R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-60940R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-60940R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD15\/SSEA1 Monoclonal Antibody, Unconjugated(bsm-60940R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60940r-100ul

Applications: FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated