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Catalog #1: bs-100631c-100ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-100628c-50ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Lane 1: mouse kidney lysates Lane 2: mouse lung lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-2602R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
Lane 1: HepG2 cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Rat Lung lysates probed with IL10RB Polyclonal Antibody, Unconjugated (bs-2602R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2602R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21981R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colitis labeled with Anti-IL-12 Polyclonal Antibody, Unconjugated (bs-0767R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Liver; Lane 2: Mouse Spleen lysates; Lane 3: NIH'3T3; Lane 4: RAW246.7 lysates probed with IL12 Polyclonal Antibody, Unconjugated (bs-0767R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spleen lysates; Lane 2: Mouse Blood cell lysates; Lane 3: Mouse RAW264.7 cell lysates; Lane 4: Mouse Liver lysates; Lane 5: Rat Spleen lysates; Lane 6: Rat Liver lysates probed with IL-12 alpha Polyclonal Antibody, Unconjugated (bs-0767R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0767R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAPI staining
U251 lysates probed with IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1789R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-12 Polyclonal Antibody, Unconjugated (bs-14637R) at 1:400 overnight at 4\u00b0C, DAB staining.
Rat Liver lysates probed with IL-12 Polyclonal Antibody, Unconjugated (bs-14637R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-14637R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Mouse spleen lysates probed with Rabbit Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL-12 beta Polyclonal Antibody, Unconjugated (bs-10641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-10641R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

HUVEC cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IL12RB1 Polyclonal Antibody(bs-2603R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
RAW264.7 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IL12RB1 Antibody(bs-2603R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-2603R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2604R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-IL-13 Polyclonal Antibody, Unconjugated (bs-0560R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-13 Polyclonal Antibody, Unconjugated (bs-0560R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0560R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-90271r-50uL

Applications: WB, FCM, IC

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with IL13 Receptor alpha 1 (1C1) Monoclonal Antibody (bsm-54211R) at 1:200 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL13RA1 Monoclonal Antibody, Unconjugated(bsm-54211R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL13RA1 Monoclonal Antibody, Unconjugated(bsm-54211R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with IL13RA1 monoclonal antibody, unconjugated (bsm-54211R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-54211r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2461R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-13Ra2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human U251 cell lysates; Lane 2: Human HeLa cell lysates probed with IL13RA2 Polyclonal Antibody, Unconjugated (bs-22785R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-22785R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22784R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin-embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL15RA) Polyclonal Antibody, Unconjugated (bs-2605R) at 1:200 overnight at 4\u00b0C, followed a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-15RA Polyclonal Antibody, Unconjugated (bs-2605R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2605R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat stomach tissue labeled with Anti-IL-17\/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) at 1:600 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat tongue tissue labeled with Anti-IL-17\/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) at 1:600 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Rat Lymph node tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Rat Thymus tissue lysates probed with IL17A Polyclonal Antibody, Unconjugated (bs-1183R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1183R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-IL-17 Polyclonal Antibody, Unconjugated(bs-2140R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: MOLT-4 cell lysates; Lane 2: U937 cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raji cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates probed with IL-17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2140R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat lung lysates Lane 2: rat pancreas lysates probed with Anti IL-17C Polyclonal Antibody, Unconjugated (bs-2611R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 22kD. Observed band size: 22kD
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IL-17C Polyclonal Antibody, Unconjugated (bs-2611R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin mouse spleen labeled with Anti-IL-17C Polyclonal Antibody, Unconjugated (bs-2611R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nIL-17C Polyclonal Antibody, Unconjugated (bs-2611R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2611R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2613R

Applications: WB, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Unconjugated

Mouse spleen lysates probed with IL17E Polyclonal Antibody, Unconjugated (bs-10943R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Lung Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Anti-IL17E Polyclonal Antibody, Unconjugated (bs-10943R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-10943R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Raw264.7 cell lysates probed with IL17RA\/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Raw264.7 cell lysates probed with \r\nIL17RA\/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2606R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62231r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

L1 rat kidney lysates and L2 rat brain lysates lysates probed with Anti- IL-17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band: 33kD\/55kD Observed band size: 35kD
Image kindly submitted by Dr. Kang Chen from Wayne University via the Bioss Discovery Program.\\n\\nPositive staining with Human Peripheral Mononuclear Cells using Rabbit Anti-IL-17RB Polyclonal Antibody, ALEXA FLUOR 488 Conjugated (bs-2610R-A488)
Lane 1: Mouse Liver lysates probed with IL-17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Uterus tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human MCF-7 cell lysates probed with IL17RB Polyclonal Antibody, Unconjugated (bs-2610R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2610R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23929R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2607R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: IL18 knockout (KO) HeLa cell lysates probed with IL18 Polyclonal Antibody, Unconjugated (bs-55111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse lung lysates probed with IL18 Polyclonal Antibody, Unconjugated (bs-55111R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IL18 ) polyclonal Antibody, Unconjugated (bs-55111R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: U2-OScell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IL18 ) polyclonal Antibody, Unconjugated (bs-55111R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55111R

Applications: WB, ELISA, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0529r

Applications: IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Normal HeLa cell lysates; Lane 2: HeLa cell treated with Brefeldin A (5 \u03bc g\/ml, 15 min) lysates;Lane 3: Jurkat cell lysates (Negative control ) probed with IL18 Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (IL18) Monoclonal Antibody, Unconjugated (bsm-56205R) at 1:250 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-IL18 antibody (bsm-56205R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-56205r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4040R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2616R

Applications: WB, ELISA, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51186M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0445R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11855R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51184M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1959R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1834R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-2621R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-IL-21R Polyclonal Antibody, Unconjugated (bs-2622R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus lysates probed with IL-21R Polyclonal Antibody, Unconjugated (bs-2622R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2622R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse splenocytes probed with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Rat testis lysates; probed with IL-22BP\/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-2625R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: mouse liver lysates Lane 2: mouse intestine probed with Rabbit Anti-IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse placenta lysates probed with IL-22R Polyclonal Antibody, Unconjugated (bs-2624R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2624R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-10576R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62788r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Antigen: bs-2711P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti IL-27R\/TCCR Polyclonal Antibody, Unconjugated (bs-2711R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse splenocytes probed with Rabbit Anti-IL-27RA Polyclonal Antibody, Unconjugated (bs-2711R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).

Catalog #1: bs-2711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-20896r-100ul

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Rabbit Anti-IL-28A\/IFNL2 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-2627R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Rabbit Anti-IL-28A\/IFNL2 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-2627R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Pancreas tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human HeLa cell lysates probed with IL28A Polyclonal Antibody, Unconjugated (bs-2627R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2627R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
SR cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with CD25 .Antibody(bsm-33113M)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bsm-33113m-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated