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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-IL2RA Polyclonal Antibody, Unconjugated (bs-0577R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: EC9706 lysates; Lane 2: mouse lymph node lysates probed with IL2RA Polyclonal Antibody, Unconjugated (bs-0577R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0577R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2631R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2632R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-62377r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Raji lysates probed with IL33 Polyclonal Antibody, Unconjugated (bs-2208R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (bs-2208R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2208R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Mouse splenocytes probed with Rabbit Anti-IL-33 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2633R-PE-Cy5) (green) at 1:100 for 40 minutes compared to control cells (blue).
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Anti \u2013IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-33 Polyclonal Antibody, Unconjugated (bs-2633R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2633R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62567r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse skin lysates probed with Rabbit Anti-IL34 Polyclonal Antibody, Unconjugated (bs-18170R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (IL34) Polyclonal Antibody, Unconjugated (bs-18170R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-18170R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-IL-3R alpha\/CD123 Polyclonal Antibody, Unconjugated (bs-2600R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with IL-3R alpha Polyclonal Antibody, Unconjugated (bs-2600R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-2600R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3689R

Applications: WB, ELISA, FCM, IHC-P

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2601R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62790r

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60284M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with IL-6R Beta\/CD130\/gp130 Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human Raji cells probed with IL-6R beta Polyclonal Antibody, Unconjugated (bs-1459R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-1459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat lung lysates Lane 2: human colon carcinomalysates probed with AntiIL-7Ra\/CD127 Polyclonal Antibody, Unconjugated (bs-1540R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.\\n
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Mouse Lymph node tissue lysates; Lane 4: Mouse Lung tissue lysates; Lane 5: Rat Spleen tissue lysates; Lane 6: Rat Thymus tissue lysates; Lane 7: Human K562 cell lysates; Lane 8: Human U-2 OS cell lysates; Lane 9: Human U-87 MG cell lysates probed with IL-7Ra Polyclonal Antibody, Unconjugated (bs-1540R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Catalog #1: bs-1540R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL7R\/CD127 Monoclonal Antibody, Unconjugated (bsm-43043M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with IL7R Monoclonal Antibody, Unconjugated(ascites of bsm-43043M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, SP-0024) and DAB (C-0010) staining.

Catalog #1: bsm-43043m-100ul

Applications: IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-98768c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-88538c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98341c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Mouse pancreas Liver lysates probed with ILDR1 Polyclonal Antibody, Unconjugated (bs-10113R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat prostate lysates probed with ILDR1 Polyclonal Antibody, Unconjugated (bs-10113R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11013R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-91909c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-100361c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ILF2 antibody (bsm-61867R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ILF2) monoclonal Antibody, unconjugated (bsm-61867R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF2 Monoclonal Antibody, Unconjugated (bsm-61867R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF2 Monoclonal Antibody, Unconjugated (bsm-61867R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61867r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (bsm-61910R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (bsm-61910R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (bsm-61910R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ILF3 Monoclonal Antibody, Unconjugated (bsm-61910R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61910r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-86397c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-86398c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82658c-100ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-75039c-100ug

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60531m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61287r-100ul

Applications: WB, FCM, IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ILK-1 Polyclonal Antibody (bs-0317R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 human colon carcinoma lysates probed with Anti ILK-1 Polyclonal Antibody, Unconjugated (bs-0317R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 50kD. Observed band size:50kD.\\n
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-0317R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-94925c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-99196c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-16605r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-92863c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90380c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82187c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87675c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-86121c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-6870R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1674R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-93286c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

HepG2 lysates probed with ILVBL Polyclonal Antibody, Unconjugated (bs-11014R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ILVBL Antibody (bs-11014R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Hcclm3 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: A431 cell lysates probed with ILVBL Polyclonal Antibody, Unconjugated (bs-11014R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-11014R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-83094c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-98637c-1mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-90752c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-82972c-5mg

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-87758c-1ml10mmin-dmso

Applications: Life Science

Reactivity:

Conjugate: Unconjugated