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Catalog #1: bs-3768R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3768R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3768R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-3768R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3768R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-3768R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3768R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3768R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3768R-PE-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bs-3768R-PE-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bs-3768R-PE-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bs-3768R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-3768R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3768R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Histone H3 (tri methyl K4) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3 (tri methyl K4)) Monoclonal Antibody, Unconjugated (bsm-33110M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33110m

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bsm-33110M-BF350

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33110M-BF405

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33110M-BF488

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33110M-BF555

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33110M-BF594

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33110M-BF647

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33110M-BF680

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33110M-BF750

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33110M-APC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC

Catalog #1: bsm-33110M-APC-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC-Cy5.5

Catalog #1: bsm-33110M-APC-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: APC-Cy7

Catalog #1: bsm-33110M-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Catalog #1: bsm-33110M-Cy3

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bsm-33110M-Cy5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bsm-33110M-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5

Catalog #1: bsm-33110M-Cy7

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bsm-33110M-FITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bsm-33110M-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bsm-33110M-PE

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE

Catalog #1: bsm-33110M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE-Cy5

Catalog #1: bsm-33110M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE-Cy5.5

Catalog #1: bsm-33110M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Others

Conjugate: PE-Cy7

Catalog #1: bsm-33110M-PerCP

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: PerCP

Catalog #1: bsm-33110M-PerCP-Cy5.5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33110m-percp-cy7-100ul

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: PerCP-Cy7

Catalog #1: bsm-33110M-RBITC

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: RBITC

Western blot was performed on whole cell (25 \u00b5g, lane 1) and histone extracts (15 \u00b5g, lane 2) from HeLa cells, and on 1 \u00b5g of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H3K9me3 (Cat. No. bs-53114R). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
HeLa cells were stained with the Bioss antibody against H3K9me3 (bs-53114R) and with DAPI. Cells were fixed with 4% formaldehyde for 10\u2019 and blocked with PBS\/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me3 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me3 (bs-53114R) in antigen-coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.
A Dot Blot analysis was performed to test the cross-reactivity of the antibody against H3K9me3 (Cat. No. bs-53114R) with peptides containing other modifications and unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.

Catalog #1: bs-53114r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-Histone H3 Polyclonal Antibody, Unconjugated(bs-0349R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse splenocytes probed with Rabbit Anti-Histone H3 Polyclonal Antibody, Unconjugated (bs-0349R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0349R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0349R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-0349R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-0349R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-0349R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-0349R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594