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Catalog #1: bsm-43176m-pe-cy7-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PE-Cy7

Catalog #1: bsm-43176m-percp-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP

Catalog #1: bsm-43176m-percp-Cy5.5-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-43176m-percp-cy7-100ul

Applications: WB

Reactivity: Bovine

Conjugate: PerCP-Cy7

Catalog #1: bsm-43176m-rbitc-100ul

Applications: WB

Reactivity: Bovine

Conjugate: RBITC

Catalog #1: v8302-1mg

Applications: ELISA, LFA

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: v8301-1mg

Applications: ELISA, LFA

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: v8303-1mg

Applications: ELISA, LFA

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bsm-72020m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-72021m

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysate; Lane 2: K562 cell lysate at 20 \u00b5g per lane probed with with bsm-51025M BRAF (125CT13.3.5) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: HL-60 Cell lysates; probed with BRAF (125CT13.3.5) Monoclonal Antibody, unconjugated (bsm-51025M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), and incubated with BRAF (125CT13.3.5) Monoclonal Antibody (bsm-51025M) at 1:25 dilution for 1 hr at 37\u2103. Followed by Alexa Fluor\u00ae 488 conjugated donkey anti-mouse antibody at 1:400 dilution for 50 min at 37\u2103. Image shows cytoplasm BRAF staining (green), and actin (red).

Catalog #1: bsm-51025M

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-34218m

Applications: WB, ELISA, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51693m

Applications: WB

Reactivity: Recombinant protein

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 4\u00b0C overnight\r\nLysate: 1: HeLa, 2: HeLa nuclear\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 120 kDa\r\nObserved MW: 120 kDa
Tissue: Human colon cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60358M

Catalog #1: bsm-60358m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BrdU (A7)) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-0917m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-0917m-bf350-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0917m-bf405-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0917m-bf488-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0917m-bf555-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0917m-bf594-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0917m-bf647-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0917m-bf680-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0917m-bf750-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0917m-apc-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC

Catalog #1: bsm-0917m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy5.5

Catalog #1: bsm-0917m-apc-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: APC-Cy7

Catalog #1: bsm-0917m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-0917m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy3

Catalog #1: bsm-0917m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5

Catalog #1: bsm-0917m-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy5.5

Catalog #1: bsm-0917m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: Cy7

Catalog #1: bsm-0917m-fitc-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: FITC

Catalog #1: bsm-0917m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Catalog #1: bsm-0917m-pe-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PE

Catalog #1: bsm-0917m-pe-cy5-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5

Catalog #1: bsm-0917m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy5.5

Catalog #1: bsm-0917m-pe-cy7-100ul

Applications: WB

Reactivity: Species independent

Conjugate: PE-Cy7

Catalog #1: bsm-0917m-percp-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP

Catalog #1: bsm-0917m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0917m-percp-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: PerCP-Cy7

Catalog #1: bsm-0917m-rbitc-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Species independent

Conjugate: RBITC

Catalog #1: bsm-60561m

Applications: WB, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51151M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60274m-100ul

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60184M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60185m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Sodium propionate (10mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa

Catalog #1: bsm-60183m

Applications: WB, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60179m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated