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Catalog #1: bsm-60188M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60182m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa\uff0c(+) HeLa+Sodium butyrate (30mM, 4hr)+Trichostatin A (500ng\/ml, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) C2C12\uff0c(+) C2C12+Sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 17 kDa\r\nObserved MW: 17 kDa

Catalog #1: bsm-60189M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60172m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60187M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60175M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60177M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60173m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-70316M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2 Monoclonal Antibody, Unconjugated (bsm-33085M) at 1:400 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with C-erbB-2\/HER2 monoclonal antibody, unconjugated (bsm-33085M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33085m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2(13H9) Monoclonal Antibody, Unconjugated (bsm-33051M) at 1:800 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with C-erbB-2\/HER2 monoclonal antibody, unconjugated (bsm-33051M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33051m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33051m-bf350-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33085m-bf350-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33051m-bf405-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33085m-bf405-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33051m-bf488-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33085m-bf488-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33051m-bf555-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33085m-bf555-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33051m-bf594-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33085m-bf594-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33051m-bf647-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33085m-bf647-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33051m-bf680-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33085m-bf680-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33051m-bf750-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33085m-bf750-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33051m-apc-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-33085m-apc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-33051m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33085m-apc-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-33051m-apc-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33085m-apc-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-33085m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33051m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33051m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33085m-cy3-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33051m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33085m-cy5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33051m-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33085m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33051m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33085m-cy7-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33051m-fitc-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33085m-fitc-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-33085m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33051m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33085m-pe-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: PE