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Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:25 overnight at 4\u00b0C, DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: T47D cell lysate probed with ALDH6A1 Monoclonal Antibody, Unconjugated (bsm-51469M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51469M

Applications: WB, IHC-P, IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-12461R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-55008R

Applications: WB, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-12461R-BF350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 350

Catalog #1: bs-12461R-BF405

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 405

Catalog #1: bs-12461R-BF488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 488

Catalog #1: bs-12461R-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 555

Catalog #1: bs-12461R-BF594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 594

Catalog #1: bs-12461R-BF647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 647

Catalog #1: bs-12461R-BF680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 680

Catalog #1: bs-12461R-BF750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor® 750

Catalog #1: bs-12461R-APC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC

Catalog #1: bs-12461R-APC-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy5

Catalog #1: bs-12461R-APC-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy5.5

Catalog #1: bs-12461R-APC-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy7

Catalog #1: bs-12461R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-12461R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-12461R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-12461R-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5.5

Catalog #1: bs-12461R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-12461R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-12461R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-12461R-PerCP

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP

Catalog #1: bs-12461R-PerCP-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP-Cy5.5

Catalog #1: bs-12461R-PerCP-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: PerCP-Cy7

Catalog #1: bs-12461R-RBITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: RBITC

Catalog #1: bs-75871C

Applications: Life Science

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at room temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: MCF7, 2: HepG2, 3: Mouse kidney, 4: Rat brain\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 1 s\r\nPredicted MW: 39 kDa\r\nObserved MW: 35 kDa
Tissue: Human colon cancer\r\nSection type: Formalin-fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60489R

Catalog #1: bsm-60489R

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ALDOB) polyclonal Antibody, Unconjugated (bs-4026R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Human HepG2 cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with ALDOB Polyclonal Antibody, Unconjugated (bs-4026R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates probed with ALDOB Polyclonal Antibody, Unconjugated (bs-4026R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4026R

Applications: WB, ELISA, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-4026R-BF350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 350

Catalog #1: bs-4026R-BF405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 405

Catalog #1: bs-4026R-BF488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 488

Catalog #1: bs-4026R-BF555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 555

Catalog #1: bs-4026R-BF594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 594

Catalog #1: bs-4026R-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 647

Catalog #1: bs-4026R-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 680

Catalog #1: bs-4026R-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor® 750

Catalog #1: bs-4026R-APC

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bs-4026R-APC-Cy5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5

Catalog #1: bs-4026R-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-4026R-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-4026R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-4026R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-4026R-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-4026R-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-4026R-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-4026R-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-4026R-HRP

Applications: WB, ELISA

Reactivity: Human

Conjugate: HRP