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Catalog #1: bsm-60374M

Applications: WB, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52728R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: 293T Cells; Lane 3: HepG2 Cells; Probed with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with 14-3-3 alpha+beta (3C5) Monoclonal Antibody (bsm-52710R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52710R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: HeLa, MCF-7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 28 kDa\r\nObserved MW: 28 kDa\r\n
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M
Tissue: esophagus\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Mouse)(sp-0024)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60449M

Catalog #1: bsm-60449M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: MCF-7 cell lysates; probed with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A431 cells were stained with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52006R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-0861M

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bsm-0861M-BF350

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 350

Catalog #1: bsm-0861M-BF405

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 405

Catalog #1: bsm-0861M-BF488

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 488

Catalog #1: bsm-0861M-BF555

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 555

Catalog #1: bsm-0861M-BF594

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 594

Catalog #1: bsm-0861M-BF647

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 647

Catalog #1: bsm-0861M-BF680

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 680

Catalog #1: bsm-0861M-BF750

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: AbBy Fluor® 750

Catalog #1: bsm-0861M-A350

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-0861M-A488

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-0861M-A555

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-0861M-A594

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-0861M-A647

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-0861M-A680

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-0861M-A750

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-0861M-APC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: APC

Catalog #1: bsm-0861M-APC-Cy5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: APC-Cy5

Catalog #1: bsm-0861M-APC-Cy5.5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: APC-Cy5.5

Catalog #1: bsm-0861M-APC-Cy7

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: APC-Cy7

Catalog #1: bsm-0861M-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-0861M-Cy3

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy3

Catalog #1: bsm-0861M-Cy5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy5

Catalog #1: bsm-0861M-Cy5.5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy5.5

Catalog #1: bsm-0861M-Cy7

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: Cy7

Catalog #1: bsm-0861M-FITC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: FITC

Catalog #1: bsm-0861M-HRP

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-0861M-PerCP

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: PerCP

Catalog #1: bsm-0861M-PerCP-Cy5.5

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0861M-PerCP-Cy7

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: PerCP-Cy7

Catalog #1: bsm-0861M-RBITC

Applications: IF(IHC-P)

Reactivity: Others

Conjugate: RBITC

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+ sodium butyrate (30mM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: NIH-3T3, 3: BRL\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa

Catalog #1: bsm-60209R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-60011M

Applications: WB

Reactivity: Human, All, Nitrotyrosine

Conjugate: Unconjugated

Western blot analysis of 4E-BP1 expression in non-transfected (A) and mouse 4E-BP1 transfected (B) 293T whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells using 4E-BP1 (3G1) Monoclonal Antibody (bsm-52007R)

Catalog #1: bsm-52007R

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52008M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-51025R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cells; Lane 2: K562 Cells; Probed with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with 5HT2C Receptor (4C3) Monoclonal Antibody (bsm-54055R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54055R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: MCF-7, 2: JAR\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 46 kDa\r\nObserved MW: 70-80\/85 kDa
Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:2000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-52422R
IP of MCF-7 cells extracts\r\nIP ab incubation condition: PTM-6392, 4\u00b0C \r\novernight, 1:100 dilution\r\nWB primary ab incubation condition: bsm-52422R, room temperature 2h, 1:1000 dilution\r\nSecondary ab: Anti-Rabbit IgG for IP (HRP)\r\nBlocking buffer and concentration: 5%\r\nNFDM\/TBST\r\nDiluting buffer and concentration: 5%\r\nNFDM\/TBST\r\n1: 5% Input\r\n2: IP with bsm-52422R\r\n3: IP with Rabbit monoclonal IgG Isotype\r\nObserved MW: 70-80\/85 kDa\r\nExposure time: 120 s

Catalog #1: bsm-52422R

Applications: WB, IHC-P, IP

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of MCF7 cells with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 lysates; Lane 2: HepG2 lysates; Line 3: A431 lysates; Probed with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52792R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cell lysates probed with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human kidney section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
NIH\/3T3 cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
HeLa cells were stained with A-RAF (24D1) Monoclonal Antibody (bsm-52015R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52015R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-70637M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: 293T, 2: HeLa, 3: HepG2\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 63 kDa\r\nObserved MW: 80 kDa\r\n
Cell line: HeLa\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nCounter stain: Tubulin (Red)\r\nComment: Color green is the positive signal for PTM-6030\r\n
Cell line: HEK-293 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4\u00b0C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for bsm-60590R\r\n

Catalog #1: bsm-60590R

Applications: WB, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ABAT\/GABA-T Monoclonal Antibody, Unconjugated (bsm-54681R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ABAT\/GABA-T Monoclonal Antibody, Unconjugated (bsm-54681R) at 1:200 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ABAT\/GABA-T Monoclonal Antibody, Unconjugated (bsm-54681R) at 1:200 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54681R

Applications: WB, IHC-P, IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated