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Catalog #1: bs-21310R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PerCP

Catalog #1: bs-2430R-PerCP

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: PerCP

Catalog #1: bs-21310R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bs-2430R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bs-21310R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: RBITC

Catalog #1: bs-2430R-RBITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: RBITC

Lane 1: A431; Lane 2: HeLa; Lane 3: HepG2; Lane 4: K562; Lane 5: PC-3 cell lysate at 20 \u00b5g per lane, probed with bsm-51448M APEX1 (1518CT337.123.86.269.232) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with APEX1 (15C2) Monoclonal Antibody (bsm-51448M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51448M

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with APEX1 Monoclonal Antibody, Unconjugated (bsm-51609M) at 1:25 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with APEX1 Monoclonal Antibody, Unconjugated (bsm-51609M) at 1:50 overnight at 4\u00b0C, DAB staining.
Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: HepG2 cell lysates; Lane 4: K562 cell lysates; Lane 5: PC-3 cell lysates probed with APEX1 Monoclonal Antibody, Unconjugated (bsm-51609M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51609m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1406R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T cell lysates; Lane 2: APEX1 knockout (KO) 293T cell lysates probed with APEX1 Polyclonal Antibody, Unconjugated (bs-55012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: HepG2 cell lysates probed with APEX1 Polyclonal Antibody, Unconjugated (bs-55012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (APEX1) polyclonal Antibody, Unconjugated (bs-55012R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-55012R

Applications: WB, IF(ICC), IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1406R-BF350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-1406R-BF405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-1406R-BF488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-1406R-BF555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-1406R-BF594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-1406R-BF647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-1406R-BF680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-1406R-BF750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-1406R-APC

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bs-1406R-APC-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-1406R-APC-Cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-1406R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1406R-Cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1406R-Cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-1406R-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-1406R-Cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-1406R-FITC

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1406R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-1406R-PE

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bs-1406R-PE-Cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bs-1406R-PE-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bs-1406R-PE-Cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bs-1406R-PerCP

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-1406R-PerCP-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-1406R-RBITC

Applications: WB

Reactivity: Human

Conjugate: RBITC

Tissue: Rat liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60707R\r\n
Tissue: Human ovarian carcinoma\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60707R\r\n
Tissue: Mouse liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60707R\r\n
Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: HEK-293, 2: HepG2, 3: NIH\/3T3, 4: \r\nRAW264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 35 kDa\r\nObserved MW: 37 kDa

Catalog #1: bsm-60707r

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human LOVO cell lysates probed with APEX2 Polyclonal Antibody, Unconjugated (bs-6587R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6587R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5991R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5991R-BF350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-350

Catalog #1: bs-5991R-BF405

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-405

Catalog #1: bs-5991R-BF488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-488

Catalog #1: bs-5991R-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-555

Catalog #1: bs-5991R-BF594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-594

Catalog #1: bs-5991R-BF647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-647

Catalog #1: bs-5991R-BF680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-680

Catalog #1: bs-5991R-BF750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: AbBy Fluor-750

Catalog #1: bs-5991R-APC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC

Catalog #1: bs-5991R-APC-Cy5.5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: APC-Cy5.5