ABCG1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1231R-Cy3-100uL |
| Product Name | ABCG1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ABCG1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9619 |
| Swiss Prot | P45844 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ABC8; WHITE1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog; ABCG1; WHT1 |
| Background | Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ABCG1 Polyclonal Antibody, Cy3 Conjugated (bs-1231R-Cy3) validated for, and at what dilution?
bs-1231R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ABCG1 Polyclonal Antibody, Cy3 Conjugated (bs-1231R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1231R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ABCG1 Polyclonal Antibody, Cy3 Conjugated (bs-1231R-Cy3)?
KLH conjugated synthetic peptide derived from human ABCG1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1231R-Cy3)?
Cy3 on bs-1231R-Cy3: excitation / emission 512,550nm/570,615nm.