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ABCG1 Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-1231R-Cy5-100uL
Product Name ABCG1 Polyclonal Antibody, Cy5 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ABCG1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 9619
Swiss Prot P45844
Subcellular location Cytoplasm, Cell membrane
Synonyms ABC8; WHITE1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; White protein homolog; ABCG1; WHT1
Background Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ABCG1 Polyclonal Antibody, Cy5 Conjugated (bs-1231R-Cy5) validated for, and at what dilution?

bs-1231R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ABCG1 Polyclonal Antibody, Cy5 Conjugated (bs-1231R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-1231R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ABCG1 Polyclonal Antibody, Cy5 Conjugated (bs-1231R-Cy5)?

KLH conjugated synthetic peptide derived from human ABCG1

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-1231R-Cy5)?

Cy5 on bs-1231R-Cy5: excitation / emission 625,650nm/670nm.