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phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Horse
Overview
Catalog # bs-14408R-Biotin-100uL
Product Name phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, Biotin Conjugated
Applications
ELISA IHC-P IHC-F
Reactivity Mouse, Rat
Predicted Reactivity Horse
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated Synthesised phosphopeptide derived from mouse Dopamine Transporter around the phosphorylation site of Thr53
Modification Site Thr53
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 13162
Swiss Prot Q61327
Subcellular location Cell membrane
Synonyms Dopamine Transporter (phospho T53); p-Dopamine Transporter (phospho T53); p-DAT1(phospho T53); DA transporter; DAT 1; DAT; SC6A3_RAT; DAT1; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 (neurotransmitter transporter), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR).
Background Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, Biotin Conjugated (bs-14408R-Biotin) validated for, and at what dilution?

bs-14408R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, Biotin Conjugated (bs-14408R-Biotin) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Horse.

How should bs-14408R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-Dopamine Transporter (Thr53) Polyclonal Antibody, Biotin Conjugated (bs-14408R-Biotin)?

KLH conjugated Synthesised phosphopeptide derived from mouse Dopamine Transporter around the phosphorylation site of Thr53

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