Phospho-DOK2 (Tyr351) Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3101R-RBITC-100uL |
| Product Name | Phospho-DOK2 (Tyr351) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is found at Tyr345 in Human. Due to the highly conserved nature of this sequence with DOK1, this antibody may react with DOK1 when it is phosphorylated at Tyr362 in Human and Tyr361 in Mouse and Rat. |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse p56Dok2 around the phosphorylation site of (Tyr351) |
| Modification Site | Tyr351 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13449 |
| Swiss Prot | O70469 |
| Subcellular location | Cytoplasm |
| Synonyms | DokR; Frip; Docking protein 2; Dok-related protein; Dok-R; Downstream of tyrosine kinase 2; IL-four receptor-interacting protein; p56(dok-2); Dok2 |
| Background | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-DOK2 (Tyr351) Polyclonal Antibody, RBITC Conjugated (bs-3101R-RBITC) validated for, and at what dilution?
bs-3101R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3101R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-DOK2 (Tyr351) Polyclonal Antibody, RBITC Conjugated (bs-3101R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from mouse p56Dok2 around the phosphorylation site of (Tyr351)
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3101R-RBITC)?
RBITC on bs-3101R-RBITC: excitation / emission 540nm/625nm.