E Tag Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2116R-Biotin-100uL |
| Product Name | E Tag Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Synonyms | GAPVPYPDPLEPR tag antibody; GAPVPYPDPLEPR epitope tag antibody; E epitope tag antibody. |
| Background | E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is E Tag Polyclonal Antibody, Biotin Conjugated (bs-2116R-Biotin) validated for, and at what dilution?
bs-2116R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E Tag Polyclonal Antibody, Biotin Conjugated (bs-2116R-Biotin) react with?
Tested reactivity: Species independent.
How should bs-2116R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E Tag Polyclonal Antibody, Biotin Conjugated (bs-2116R-Biotin)?
KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR)