E Tag Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2116R-HRP-100uL |
| Product Name | E Tag Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | GAPVPYPDPLEPR tag antibody; GAPVPYPDPLEPR epitope tag antibody; E epitope tag antibody. |
| Background | E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is E Tag Polyclonal Antibody, HRP Conjugated (bs-2116R-HRP) validated for, and at what dilution?
bs-2116R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E Tag Polyclonal Antibody, HRP Conjugated (bs-2116R-HRP) react with?
Tested reactivity: Species independent.
How should bs-2116R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E Tag Polyclonal Antibody, HRP Conjugated (bs-2116R-HRP)?
KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR)