AIF1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-42057r-cy3-100uL |
| Product Name | AIF1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant mouse AIF1 protein |
| Immunogen Range | 2-147/147 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11629 |
| Swiss Prot | O70200 |
| Subcellular location | Cytoplasm, Cell membrane |
| Background | Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-42057R-Cy3) validated for, and at what dilution?
bsm-42057R-Cy3 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-42057R-Cy3) react with?
Tested reactivity: Mouse.
How should bsm-42057R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-42057R-Cy3)?
Recombinant mouse AIF1 protein Immunogen range: 2-147/147.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-42057R-Cy3)?
Cy3 on bsm-42057R-Cy3: excitation / emission 512,550nm/570,615nm.