AIF1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-54132r-cy3-100uL |
| Product Name | AIF1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | Recombinant protein corresponding to mouse AIF1 aa 100 to the C-terminus |
| Immunogen Range | 100-147/147 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 199 |
| Swiss Prot | P55008 |
| Synonyms | Allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; AIF-1; AIF1; IBA1. |
| Background | Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. |
| Application Dilution | |
| WB | 1:200-1000 |
| IF | 1:1000-2000 |
Frequently asked questions
Which applications is AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-54132R-Cy3) validated for, and at what dilution?
bsm-54132R-Cy3 is validated for WB (1:200-1000); IF (1:1000-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-54132R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-54132R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AIF1 Recombinant Antibody, Cy3 Conjugated (bsm-54132R-Cy3)?
Recombinant protein corresponding to mouse AIF1 aa 100 to the C-terminus Immunogen range: 100-147/147.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-54132R-Cy3)?
Cy3 on bsm-54132R-Cy3: excitation / emission 512,550nm/570,615nm.