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CRISPR, Gene Editing & DNA-Repair Antibodies

CRISPR efficiency is only part of the result. Evaluate DNA-damage signaling, repair-pathway activation, and editing outcomes with antibodies targeting Cas9, RAD51, BRCA2, DNA-PK, γH2AX, ATM, ATR, and other key genome-stability proteins.



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Catalog #1: bsm-61583r-biotin

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61583r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61302r-biotin

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61302r-TR

Applications: WB, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61400r-biotin

Applications: ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Western blot analysis of Jurkat cell lysate. Using Ctip2 (bsm-61400R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ctip2 Monoclonal Antibody, Unconjugated (bsm-61400R) at 1:300 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61400r-TR

Applications: ELISA, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61562r-biotin

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with CHEK2 monoclonal antibody, unconjugated (bsm-61562R) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (bsm-61562R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (bsm-61562R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (bsm-61562R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61562r

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54560R

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: A431 Cells; Lane 3: SH-SY5Y Cells; Lane 4: SiHa Cells; Lane 5: 293 Cells; Lane 6: Hela Cells; Lane 7: HepG2 Cells; Probed with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54464R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61460r-biotin

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with Ku70 \/ XRCC6 monoclonal antibody, unconjugated (bsm-61460R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ku70 \/ XRCC6 Monoclonal Antibody, Unconjugated (bsm-61460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61460r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cleaved PARP1 p25 Monoclonal Antibody, Unconjugated (bsm-60941R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60941r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61685r-biotin

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61685r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70350M

Applications: WB, FCM, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-70352M

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated