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CRISPR, Gene Editing & DNA-Repair Antibodies

CRISPR efficiency is only part of the result. Evaluate DNA-damage signaling, repair-pathway activation, and editing outcomes with antibodies targeting Cas9, RAD51, BRCA2, DNA-PK, γH2AX, ATM, ATR, and other key genome-stability proteins.



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Catalog #1: bsm-60629r-apc-cy7

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-60629r-bf594

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60629r-bf350

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60629r-apc-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-60629r-bf750

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60629r-apc

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

25 ug total protein per lane of various lysates (see on figure) probed with RPA2 monoclonal antibody, unconjugated (bsm-60629R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPA2 Monoclonal Antibody, Unconjugated (bsm-60629R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPA2 Monoclonal Antibody, Unconjugated (bsm-60629R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPA2 Monoclonal Antibody, Unconjugated (bsm-60629R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60629r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: A431 Cells; Lane 3: SH-SY5Y Cells; Lane 4: SiHa Cells; Lane 5: 293 Cells; Lane 6: Hela Cells; Lane 7: HepG2 Cells; Probed with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54464R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Probed with RPA1 (1C2) Monoclonal Antibody (bsm-54296R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with RPA1 (1C2) Monoclonal Antibody (bsm-54296R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RPA1 (1C2) Monoclonal Antibody (bsm-54296R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RPA1 (1C2) Monoclonal Antibody (bsm-54296R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54296R

Applications: WB, FCM, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62265r-rbitc

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-62265r-percp-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-62265r-percp-cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-62265r-percp

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-62265r-pe-cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-62265r-pe-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-62265r-pe-cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-62265r-pe

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-62265r-hrp

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-62265r-fitc

Applications: WB

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-62265r-cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-62265r-cy5

Applications: WB

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-62265r-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-62265r-biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-62265r-cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-62265r-bf750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-62265r-bf680

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-62265r-bf647

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-62265r-bf594

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-62265r-bf350

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-62265r-apc

Applications: WB

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-62265r-bf555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-62265r-bf488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-62265r-apc-cy7

Applications: WB

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-62265r-bf405

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-62265r-apc-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-61028r-bf750

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61028r-bf488

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61028r-bf555

Applications: WB

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61028r-apc-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-61028r-pe-cy7

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-61028r-percp-cy7

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-61028r-pe

Applications: WB

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-61028r-cy3

Applications: WB

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-61028r-cy7

Applications: WB

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-61028r-rbitc

Applications: WB

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-61028r-percp

Applications: WB

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-61028r-percp-Cy5.5

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61028r-pe-cy5

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5