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Epitope Tag Antibodies

 

 

An epitope tag is a biological structure or sequence, recognized by an antibody, such as a protein or carbohydrate, that acts as an antigen. When no antibody is available for the protein/ molecule of interest, epitope tagging is a great alternative to allow researchers to track protein expression and localization.

The tag can be attached to a protein/molecule of interest by using chemical conjugation or genetic fusion. The tags can also improve solubility, folding, purification, and expression levels. With epitope tagging, affinity purification can be performed for the tagged molecule by using tag-specific antibodies coupled to chromatographic resins.

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Formalin-fixed and paraffin embedded rat liver labeled with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 followed by conjugation to the secondary antibody
Transfected HBX cells probed with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P) probed with EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-2194R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat, Tags

Conjugate: Unconjugated

Catalog #: bs-0965R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Lane 1: E.coli Lysate lysates probed with HA tag Polyclonal Antibody, Unconjugated (bs-0966R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HA-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 4 ug\r\nPrimary: Anti-HA tag (bs-0966R) at 1\/1000 ~ 1\/10000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution

Catalog #: bs-0966R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bsm-0287M

Applications: WB

Reactivity: Human, Tags, Others

Conjugate: Unconjugated

Antigen: bs-2110P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: E.coli Lysate lysates probed with VSV-G tag Polyclonal Antibody, Unconjugated (bs-2110R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-2110R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bs-0890R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Unconjugated

Catalog #: bs-0890R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: ALEXA FLUOR® 488

Antigen: bs-2109P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: E.coli Lysate lysates probed with V5 tag Polyclonal Antibody, Unconjugated (bs-2109R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with V5 tag Polyclonal Antibody, Unconjugated (bs-2109R) at 1:100000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-2109R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bs-2194R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat, Tags

Conjugate: HRP

Catalog #: bsm-0966M

Applications: WB, IF(ICC)

Reactivity: Tags

Conjugate: Unconjugated

Catalog #: bs-2116R-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded human cervical carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Thioredoxin Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33018P) probed with Thioredoxin\/Trx Polyclonal Antibody, Unconjugated (bs-0458R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Thioredoxin Polyclonal Antibody(bs-0458R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Thioredoxin) polyclonal Antibody, Unconjugated (bs-0458R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #: bs-0458R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #: bs-0890R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-0890R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy3

Catalog #: bs-0890R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Pig, Tags

Conjugate: HRP

Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P); Lane 2: Control (-) E.coli lysates probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GFP-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-33009P) probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:1000~10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GFP-tagged fusion protein probed with GFP Polyclonal Antibody, Unconjugated (bs-0639R) at 1:20000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-0639R

Applications: WB, IF

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bsm-0966M-Biotin

Applications: WB

Reactivity: Tags

Conjugate: Biotin

Catalog #: bsm-0966M-HRP

Applications: WB

Reactivity: Tags

Conjugate: HRP

Catalog #: bsm-33004M-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #: bs-10582R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Tags, Others

Conjugate: HRP

Catalog #: bs-0890R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: FITC

Catalog #: bs-2112R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags

Conjugate: Unconjugated

Catalog #: bs-2115R

Applications: WB, ELISA, ICC

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bs-2116R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

GFP protein lysate probed with bsm-51005M GFP Tag (8A1) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates (Cat#: bs-33009P) probed with GFP-Tag Monoclonal Antibody, Unconjugated (bsm-51005M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-51005M

Applications: WB

Reactivity: Human, Others

Conjugate: Unconjugated

Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate; Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate probed with His tag (6E6) Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with His tag Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:200000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bsm-33004M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Recombinant ORF53 [Ictalurid herpesvirus 1] Protein lysates probed with His Tag Polyclonal Antibody, Unconjugated (bs-10582R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41403P) probed with His Tag Polyclonall Antibody, Unconjugated (bs-10582R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysates (Cat#: bs-41230P) probed with His Tag Polyclonall Antibody, Unconjugated (bs-10582R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4 ug\r\nPrimary: Anti-His Tag (bs-10582R) at 1\/2000 ~ 1\/5000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution

Catalog #: bs-10582R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bs-10582R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #: bsm-33011M

Applications: WB

Reactivity: Others

Conjugate: Unconjugated

Catalog #: bsm-33011M-HRP

Applications: WB

Reactivity: Others

Conjugate: HRP

Catalog #: bs-0890R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-0890R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy5

Catalog #: bs-0890R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Pig, Tags

Conjugate: Biotin

Catalog #: bs-0966R-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #: bs-0966R-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #: bs-2110R-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Lane 1: GST-Tagged Fusion Protein Overexpression E.coli lysates; Lane 2: GST-Tagged Fusion Protein Overexpression E.coli lysates probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GST-tagged fusion protein probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4\u2103 overnight.

Catalog #: bs-2122R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #: bs-2122R-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #: bs-2194R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat, Tags

Conjugate: Biotin

Catalog #: bs-0890R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-0890R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-0890R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Pig, Tags

Conjugate: Cy7

Catalog #: bs-2109R-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #: bs-2109R-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP

Catalog #: bs-2110R-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #: bs-2113R

Applications: ELISA, ICC

Reactivity: Tags

Conjugate: Unconjugated

Catalog #: bs-2115R-Biotin

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Biotin

Catalog #: bs-2115R-HRP

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: HRP