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IF (IHC-P) Antibodies



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Catalog #1: bs-10451R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Mouse heart lysates probed with Phospho-Smad1 (Ser463) Polyclonal Antibody, Unconjugated (bs-10381R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse muscle lysates probed with Phospho-Smad1 (Ser463) Polyclonal Antibody, Unconjugated (bs-10381R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-Smad1 (Ser463) Antibody(bs-10381R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-10381R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10380R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human myometrium labeled with Anti-phospho-AKT1 (Tyr474)Polyclonal Antibody, Unconjugated (bs-10133R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
Mouse lung lysates probed with AKT1\/2\/3 (Tyr474) Polyclonal Antibody, unconjugated (bs-10133R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n

Catalog #1: bs-10133R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0894R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0955R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0955R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-10118R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0036R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-5209r-cy55

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-5209r-percp-cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-5209r-percp

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-5209r-cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-5209r-apc-cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-5209r-rbitc

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-5209r-percp-cy55

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-5209r-fitc

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-5209r-cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-5209r-bf405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-5209r-cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-5209r-bf488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-5209r-bf555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-5209r-bf750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-5209r-bf350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Catalog #1: bs-5209r-apc

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-5209r-bf680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-5209r-bf594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-5209r-pe

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-5209r-bf647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-5209r-apc-cy55

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-AKT3 (Ser472)Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining
Lane 1: Human HUVEC cell lysates; Lane 2: Human A431 cell lysates probed with AKT1\/2\/3 Polyclonal Antibody, Unconjugated (bs-5209R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-5209R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5209r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7044r-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Catalog #1: bs-7044r-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-7044r-percp

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP

Catalog #1: bs-7044r-percp-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bs-7044r-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-7044r-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy7

Catalog #1: bs-7044r-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-7044r-pe

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-7044r-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-7044r-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-7044r-bf750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-750

Catalog #1: bs-7044r-bf680

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-680

Catalog #1: bs-7044r-bf488

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-488

Catalog #1: bs-7044r-bf594

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-594

Catalog #1: bs-7044r-bf647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-647

Catalog #1: bs-7044r-bf555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-555

Catalog #1: bs-7044r-bf350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-350