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IF (IHC-P) Antibodies



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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-Mst1(Thr183) Polyclonal Antibody, Unconjugated (bs-4635R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti Phospho-Mst1(Thr183) Polyclonal Antibody, Unconjugated (bs-4635R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 56kD. Observed band size:56kD.\\n
Antigen: bs-4635P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-MST1 (Thr183)) polyclonal Antibody, Unconjugated (bs-4635R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-4635r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-3741r-percp-cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bs-3741r-rbitc

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-3741r-percp

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-3741r-percp-cy55

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3741r-pe

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-3741r-fitc

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-3741r-cy55

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-3741r-cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-3741r-cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-3741r-bf750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bs-3741r-bf680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bs-3741r-cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3741r-bf555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bs-3741r-apc

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bs-3741r-bf594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bs-3741r-bf647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bs-3741r-bf488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bs-3741r-apc-cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bs-3741r-bf405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bs-3741r-bf350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti phospho-HSF1(Ser326)Polyclonal Antibody, Unconjugated (bs-3741R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-HSF1(Ser326)Polyclonal Antibody, Unconjugated (bs-3741R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3741r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3741r-apc-cy55

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (c-Abl (Tyr226) ) Polyclonal Antibody, Unconjugated (bs-3041R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (c-Abl (Tyr226) ) Polyclonal Antibody, Unconjugated (bs-3041R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (c-Abl (Tyr226) ) Polyclonal Antibody, Unconjugated (bs-3041R ) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3041r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-1651r-percp

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP

Catalog #1: bs-1651r-percp-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy7

Catalog #1: bs-1651r-percp-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PerCP-Cy5.5

Catalog #1: bs-1651r-bf488

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-488

Catalog #1: bs-1651r-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: RBITC

Catalog #1: bs-1651r-fitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-1651r-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-1651r-bf405

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-405

Catalog #1: bs-1651r-cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-1651r-bf555

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-555

Catalog #1: bs-1651r-pe

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-1651r-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-1651r-bf594

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-594

Catalog #1: bs-1651r-bf647

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-647

Catalog #1: bs-1651r-cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-1651r-bf750

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-750

Catalog #1: bs-1651r-bf680

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-680

Catalog #1: bs-1651r-bf350

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor-350

Catalog #1: bs-15963r-rbitc

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bs-1651r-apc-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy5.5

Catalog #1: bs-15963r-apc-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Lane 1: Mouse Small intestine lysates; Lane 2: Mouse Large intestine lysates probed with AKT1\/3 (Tyr473) Polyclonal Antibody, Unconjugated (bs-15963R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-AKT3 (Tyr473) . Antibody(bs-15963R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-AKT3 (Tyr473)) polyclonal Antibody, Unconjugated (bs-15963R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-15963r

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1651r-apc-cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy7

Catalog #1: bs-15963r-percp-cy55

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5