Get 20% off all conjugated antibody purchases with code "CON-ANTIBODY2025"! Offer valid on direct orders only. Available for US customers and internationally through distributors.

Human CD80 Ready-To-Use IHC Kit

Immunohistochemical analysis of paraffin embedded human tonsil tissue slide using IHC0231H (Human CD80 IHC Kit).
Immunohistochemical analysis of paraffin embedded human lymph nodes tissue slide using IHC0231H (Human CD80 IHC Kit).
Immunohistochemical analysis of paraffin embedded human spleen tissue slide using IHC0231H (Human CD80 IHC Kit).
Immunohistochemical analysis of paraffin embedded human thymus tissue slide using IHC0231H (Human CD80 IHC Kit).

Human CD80 Ready-To-Use IHC Kit

Cat. No.: IHC0231H
Sample Type:
FFPE tissue
Size:
50T (including a control slide)
Storage and Stability:
Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

Synonyms

B7; B7-1; B71; Cd28l; Cd80; Ly-53; Ly53; MIC17; TSA1; Activation B7-1 antigen; B lymphocyte activation antigen B7; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80.

Materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human CD80 Mouse mAb) 6ml RTU 2-8°C
6 Secondary Antibody (AffiniPure Goat Anti-Mouse IgG H&L (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)/HRP) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human tonsil) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.


As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.