Human CD80 Ready-To-Use IHC Kit
Human CD80 Ready-To-Use IHC Kit
Cat. No.: IHC0231HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Synonyms
B7; B7-1; B71; Cd28l; Cd80; Ly-53; Ly53; MIC17; TSA1; Activation B7-1 antigen; B lymphocyte activation antigen B7; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80.
Materials Supplied
Number | Component | 50T | Concentration | Storage |
---|---|---|---|---|
1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
4 | Blocking Buffer | 3ml | RTU | 2-8°C |
5 | Primary Antibody (Human CD80 Mouse mAb) | 6ml | RTU | 2-8°C |
6 | Secondary Antibody (AffiniPure Goat Anti-Mouse IgG H&L (min X Hu, Bov, Hrs, Rb, Sw Sr Prot)/HRP) | 6ml | RTU | 2-8°C |
7 | Chromogen Component A | 0.3ml | RTU | -20°C |
8 | Chromogen Component B | 0.3ml | RTU | -20°C |
9 | Counter Staining Reagent | 5ml | RTU | RT |
10 | Differentiation Reagent | 6ml | RTU | RT |
11 | Mounting Media | 5ml | RTU | RT |
12 | Control slide (Human tonsil) | 1 slide | RTU | RT |
13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.