Human AIF Ready-To-Use IHC Kit
Human AIF Ready-To-Use IHC Kit
Cat. No.: IHC0318HSample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
Background
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
Synonyms
AIF, AIFM1, AIFsh2, CMT2D, CMTX4, COWCK, COXPD6, DFNX5, Hq, NADMR, NAMSD, PDCD8.Validation Data
Materials Supplied
| Number | Component | 50T | Concentration | Storage |
|---|---|---|---|---|
| 1 | PBS Buffer (powder) | 2L x 2 | 20x | RT |
| 2 | Antigen Retrieval Buffer | 20ml | 100x | 2-8°C |
| 3 | Endogenous Peroxidase Blocking Buffer | 3ml | RTU | 2-8°C |
| 4 | Blocking Buffer | 3ml | RTU | 2-8°C |
| 5 | Primary Antibody (Human AIF Recombinant Rabbit mAb) | 6ml | RTU | 2-8°C |
| 6 | Secondary Antibody (Goat Anti-Rabbit IgG H&L, HRP conjugated) | 6ml | RTU | 2-8°C |
| 7 | Chromogen Component A | 0.3ml | RTU | -20°C |
| 8 | Chromogen Component B | 0.3ml | RTU | -20°C |
| 9 | Counter Staining Reagent | 5ml | RTU | RT |
| 10 | Differentiation Reagent | 6ml | RTU | RT |
| 11 | Mounting Media | 5ml | RTU | RT |
| 12 | Control slide (Human liver) | 1 slide | RTU | RT |
| 13 | Datasheet | 1 copy |
Notes
- The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
- Do not allow slides to dry at any time during this procedure.
- Please don't replace the matching reagents in this product with other manufacturers' products.
- As DAB is a carcinogen, please take necessary precautions.
- PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.
As DAB is a carcinogen, please take necessary precautions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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