Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Human FUT4 Ready-To-Use IHC Kit

Immunohistochemical analysis of paraffin embedded Human placenta tissue slide using IHC0686H (Human FUT4 Kit).
Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue slide using IHC0686H (Human FUT4 Kit).
Immunohistochemical analysis of paraffin embedded Human lung cancer tissue slide using IHC0686H (Human FUT4 Kit).
Immunohistochemical analysis of paraffin embedded Human lymph nodes tissue slide using IHC0686H (Human FUT4 Kit).
Immunohistochemical analysis of paraffin embedded Human spleen tissue slide using IHC0686H (Human FUT4 Kit).
Immunohistochemical analysis of paraffin embedded Human kidney tissue slide using IHC0686H (Human FUT4 Kit).

Human FUT4 Ready-To-Use IHC Kit

Cat. No.: IHC0686H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Synonyms

LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; FCT3A; Fucosyltransferase 4; Fucosyltransferase IV; FUT4; SSEA 1.Validation Data

Materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human FUT4 Mouse mAb) 6ml RTU 2-8°C
6 Secondary Antibody (AffiniPure Goat Anti-Mouse IgG H&L, HRP conjugated) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human placenta) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.


As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Search our catalog of hundreds of thousands of products