FURA-2 pentapotassium salt

Overview
Catalog #	bs-76292c-1mg
Product Name	FURA-2 pentapotassium salt
Specifications
Storage Buffer	Solid
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 113694-64-7 Molecular formula: C29H22N3O14 . 5K Molecular weight: 831.99 Purity: >90% (HPLC) Appearance: Off-white solid to light yellow solid. Solubility: Soluble in water or DMSO. InChiKey: HQKWBBCTCLUGSX-UHFFFAOYSA-I SMILES: [O-]C(C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC([O-])=O)CC([O-])=O)C(OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=C3)=O.[K+].[K+].[K+].[K+].[K+] SMILES: [K+].[K+].[K+].[K+].[K+].CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC2=C(C=C3OC(=CC3=C2)C2=NC=C(O2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1
Description	Fura-2 pentapotassium salt is a widely used UV-excitable fluorescent calcium indicator. It is a fluorophore chelating probe for Ca2+ used widely in biological systems to analyze intracellular calcium levels. It can be used to detect calcium in cells. A diagnostic shift in the excitation spectrum from 363nm to 335nm indicates FURA-2 chelation to Ca2+. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. Analysis of Ca2+ flux in and out of cells with Fura-2 introduces a powerful spectrographic handle to investigate and elucidate correlated biological events such as neuronal ion channel activation. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels. It can also be used as a zinc indicator or measuring membrane potential. Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Fura-2 potassium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode. However, it may be loaded into cells via microinjection or scrape loading. Spectral data: lambdaEx 363nm, lambdaEm 512nm in water.