Fluorescein diacetate

Overview
Catalog #	bs-76300c-10g
Product Name	Fluorescein diacetate
Specifications
Storage Buffer	Powder
Storage Condition	Stable for 2 years after receipt when stored at -20°C.
Target
Product Information	CAS Number: 596-09-8 Molecular formula: C24H16O7 Molecular weight: 416.38 Purity: >95% (NMR) Appearance: White to off-white powder. Solubility: Soluble in DMSO, ethanol, water, chloroform or acetone (25mg/ml). InChiKey: CHADEQDQBURGHL-UHFFFAOYSA-N SMILES: O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC(C)=O)C=C4)OC5=CC(OC(C)=O)=CC=C53
Description	FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: lambdaex=494nm, lambdaem=521nm.