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Monoclonal IHC Antibodies



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Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX8 Monoclonal Antibody, Unconjugated(bsm-52737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX8 Monoclonal Antibody, Unconjugated(bsm-52737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat thyroid gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX8 Monoclonal Antibody, Unconjugated(bsm-52737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX8 Monoclonal Antibody, Unconjugated(bsm-52737R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52737r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH\/3T3 Cells; Lane 2: Hela Cells; Probed with SMC1 (3A7) Monoclonal Antibody (bsm-52649R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SMC1 (3A7) Monoclonal Antibody (bsm-52649R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SMC1 (3A7) Monoclonal Antibody (bsm-52649R) at 1:300 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SMC1 (3A7) Monoclonal Antibody (bsm-52649R) at 1:300 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52649R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: MCF-7 Cells; Lane 3: NIH\/3T3 Cells; Probed with Histone H3 (3C3) Monoclonal Antibody (bsm-52751R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (3C3) Monoclonal Antibody (bsm-52751R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Histone H3 (3C3) Monoclonal Antibody (bsm-52751R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Histone H3 (3C3) Monoclonal Antibody (bsm-52751R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52751R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 Cells; Lane 2: HUVEC Cells; Probed with UBC9 (6C10) Monoclonal Antibody (bsm-52500R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C
IF(ICC) staining with UBC9 (6C10) Monoclonal Antibody (bsm-52500R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with UBC9 (6C10) Monoclonal Antibody (bsm-52500R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with UBC9 (6C10) Monoclonal Antibody (bsm-52500R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52500R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: K562 cells lysates; Probed with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n\r\n
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:200 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52945R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of sw480 cells with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100 in SKOV-3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with HE4 (8C3) Monoclonal Antibody (bsm-52840R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human A431 cell lysates probed with HE4 Monoclonal Antibody, Unconjugated (bsm-52840R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52840r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Rat brain lysates; Lane 2: Mouse kidney lysates; Probed with Calbindin (8C4) Monoclonal Antibody (bsm-52834R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with Calbindin (8C4) Monoclonal Antibody (bsm-52834R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Calbindin (8C4) Monoclonal Antibody (bsm-52834R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Calbindin (8C4) Monoclonal Antibody (bsm-52834R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52834R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Histone H3 (6C3) Monoclonal Antibody (bsm-52769R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: NIH\/3T3 Cells; Lane 3: MCF-7 Cells; Probed with Histone H3 (6C3) Monoclonal Antibody (bsm-52769R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H3 (6C3) Monoclonal Antibody (bsm-52769R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Histone H3 (6C3) Monoclonal Antibody (bsm-52769R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52769R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

SW480 cells Probed with Sumo 2+3 (7G10) Monoclonal Antibody (bsm-52716R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Sumo 2+3 (7G10) Monoclonal Antibody (bsm-52716R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Sumo 2+3 (7G10) Monoclonal Antibody (bsm-52716R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Sumo 2+3 (7G10) Monoclonal Antibody (bsm-52716R) at 1:100 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52716R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CCNA2 Monoclonal Antibody, Unconjugated (bsm-52660R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CCNA2 Monoclonal Antibody, Unconjugated (bsm-52660R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CCNA2 Monoclonal Antibody, Unconjugated (bsm-52660R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CCNA2 Monoclonal Antibody, Unconjugated (bsm-52660R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52660r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Hela cell lysate probed with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52658R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of MCF7 cells with Cdk4 (6A4) Monoclonal Antibody (bsm-52651R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Hela cells Probed with Cdk4 (6A4) Monoclonal Antibody (bsm-52651R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cdk4 (6A4) Monoclonal Antibody (bsm-52651R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cdk4 (6A4) Monoclonal Antibody (bsm-52651R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52651R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human brain Probed with Kv1.1 potassium channel (5B8) Monoclonal Antibody (bsm-52626R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with Kv1.1 potassium channel (5B8) Monoclonal Antibody (bsm-52626R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52626R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with hnRNP C1+C2 (9F7) Monoclonal Antibody (bsm-52580R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: MCF-7 Cells; Lane 3: HepG2 Cells; Probed with hnRNP C1+C2 (9F7) Monoclonal Antibody (bsm-52580R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with hnRNP C1+C2 (9F7) Monoclonal Antibody (bsm-52580R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with hnRNP C1+C2 (9F7) Monoclonal Antibody (bsm-52580R) at 1:300 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52580R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with GAP43 Monoclonal Antibody, Unconjugated(bsm-52565R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52565r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Human skeletal muscle lysate probed with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Adiponectin receptor protein 1 (5G11) Monoclonal Antibody (bsm-52557R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52557R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

A549 cells lysates probed with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PGP9.5 (2A1) Monoclonal Antibody (bsm-52897R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52897R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Thymus tissue lysates probed with CD90\/Thy-1 Monoclonal Antibody, Unconjugated (bsm-52869R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90\/Thy-1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90\/Thy-1 Monoclonal Antibody, Unconjugated(bsm-52869R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52869r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cells; Lane 2: NIH\/3T3 Cells; Lane 3: 293T Cells; Probed with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52611R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PYK2) monoclonal Antibody, unconjugated (bsm-52553R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with PYK2 monoclonal antibody, unconjugated (bsm-52553R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PYK2 Monoclonal Antibody, Unconjugated (bsm-52553R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PYK2 Monoclonal Antibody, Unconjugated (bsm-52553R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52553r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: MCF-7 Cells; Probed with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DIABLO (9A7) Monoclonal Antibody (bsm-52544R) at 1:100 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52544R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Hela cells with PABPN1 (1C1) Monoclonal Antibody(bsm-52941R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
MCF-7 cells lysates probed with PABPN1 (1C1) Monoclonal Antibody (bsm-52941R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with PABPN1 (1C1) Monoclonal Antibody (bsm-52941R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PABPN1 (1C1) Monoclonal Antibody (bsm-52941R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52941R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-52934R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with Integrin linked ILK (3G2) Monoclonal Antibody (bsm-52546R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: 293T Cells; Probed with Integrin linked ILK (3G2) Monoclonal Antibody (bsm-52546R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Integrin linked ILK (3G2) Monoclonal Antibody (bsm-52546R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Integrin linked ILK (3G2) Monoclonal Antibody (bsm-52546R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52546R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HepG2; Lane 2: Rat brain; Lane 3: SW480 Cells; Probed with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MEKK2 (9A2) Monoclonal Antibody (bsm-52540R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52540R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: MCF-7 cells lysates; Probed with POLR2A (Ser5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells with POLR2A (Ser5) (12C8) Monoclonal Antibody (bsm-52962R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Phospho-POLR2A (S5) (12C8) Monoclonal Antibody (bsm-52962R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52962R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: MCF-7 cells lysates; Probed with POLR2A (Ser2) (1C1) Monoclonal Antibody (bsm-52961R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with POLR2A (Ser2) (1C1) Monoclonal Antibody (bsm-52961R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Phospho-POLR2A (S2) (1C1) Monoclonal Antibody (bsm-52961R) at 1:200 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Phospho-POLR2A (S2) (1C1) Monoclonal Antibody (bsm-52961R) at 1:200 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52961R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat brain cells lysates probed with mGluR1 (2C1) Monoclonal Antibody (bsm-52959R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with mGluR1 (2C1) Monoclonal Antibody (bsm-52959R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with mGluR1 (2C1) Monoclonal Antibody (bsm-52959R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52959R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat brain cells lysates probed with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NUR77 (1C1) Monoclonal Antibody (bsm-52972R) at 1:100 in NIH-3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52972r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse heart cells lysates probed with CD26 (4A9) Monoclonal Antibody (bsm-52968R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n
IF(ICC) staining with CD26 (4A9) Monoclonal Antibody (bsm-52968R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD26 (4A9) Monoclonal Antibody (bsm-52968R) at 1:100 in PC-12 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52968R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Jurkat cells with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Jurkat Cells; Lane 2: MCF-7 Cells; Probed with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with eIF5A (3A6) Monoclonal Antibody (bsm-52519R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52519R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells lysates; Lane 2: A431 cells lysates; Line 3: HepG2 cells lysates; Probed with Cyclophilin A (1C6) Monoclonal Antibody (bsm-52908R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Cyclophilin A (1C6) Monoclonal Antibody (bsm-52908R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Cyclophilin A (1C6) Monoclonal Antibody (bsm-52908R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Cyclophilin A (1C6) Monoclonal Antibody (bsm-52908R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52908R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

A431 cell lysate probed with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with PRMT5 (28A3) Monoclonal Antibody (bsm-52463R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52463R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Caspase-1 monoclonal antibody, unconjugated (bsm-52441R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The THP-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Caspase-1 antibody (bsm-52441R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-52441r-100ul

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Unconjugated

Hela cell lysates probed with Calponin (19A1) Monoclonal Antibody, Unconjugated (bsm-52358R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with Calponin (19A1) Monoclonal Antibody (bsm-52358R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with Calponin (19A1) Monoclonal Antibody (bsm-52358R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse bladder tissue incubated with Calponin (19A1) Monoclonal Antibody (bsm-52358R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52358R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: Raji cell lysates probed with FUBP1 (3F12) Monoclonal Antibody, Unconjugated (bsm-52344R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Flow cytometric analysis of Jurkat cells with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52344R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: K562 lysate; Probed with PUMA (2G6) Monoclonal Antibody, Unconjugated (bsm-52276R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.\r\n
Flow cytometric analysis of HeLa cells with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse small intestine tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52276R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY-5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: HepG2 Cells; Lane 9: A549 Cells; Probed with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with SFRS3 (9C11) Monoclonal Antibody (bsm-54467R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54468R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Heart; Probed with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54465R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse testis; Lane 2: A431 Cells; Lane 3: SH-SY5Y Cells; Lane 4: SiHa Cells; Lane 5: 293 Cells; Lane 6: Hela Cells; Lane 7: HepG2 Cells; Probed with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with RPA2 (4F8) Monoclonal Antibody (bsm-54464R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54464R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse skeletal muscle; Lane 2: Rat brain; Lane 3: A431 Cells; Lane 4: SH-SY5Y Cells; Lane 5: SiHa Cells; Lane 6: 293 Cells; Lane 7: Hela Cells; Lane 8: PC-3M Cells; Lane 9: HepG2 Cells; Lane 10: A549 Cells; Probed with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with PPP1CB (6G6) Monoclonal Antibody (bsm-54463R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Catalog #1: bsm-54463R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SH-SY5Y Cells; Probed with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse Embryo tissue incubated with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Flow cytometric analysis of A549 cells with TBXT (1F11) Monoclonal Antibody (bsm-54459R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54459R

Applications: WB, FCM, IHC-P, IF(ICC), IP

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse Spleen; Probed with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with PSENEN (1D8) Monoclonal Antibody (bsm-54452R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54452R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARK2 (1F10)) Monoclonal Antibody, Unconjugated (bsm-54447R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MARK2 (1F10)) Monoclonal Antibody, Unconjugated (bsm-54447R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-54447r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293 Cells; Probed with Exportin-5 (4H7) Monoclonal Antibody (bsm-54445R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Exportin-5 (4H7) Monoclonal Antibody (bsm-54445R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with Exportin-5 (4H7) Monoclonal Antibody (bsm-54445R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with Exportin-5 (4H7) Monoclonal Antibody (bsm-54445R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54445R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: HL-60 Cells; Probed with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54442R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A431 Cells; Lane 2: A549 Cells; Lane 3: Rat Brain; Lane 4: Mouse Brain; Probed with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Jurkat cells with PGAM1 (2G9) Monoclonal Antibody (bsm-54440R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Catalog #1: bsm-54440R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: MCF-7 Cells; Probed with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Lysyl-tRNA synthetase (1H8) Monoclonal Antibody (bsm-54437R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54437R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated