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Catalog #1: bs-0042R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0042R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-1495R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
293T cells probed with Occludin Polyclonal Antibody, unconjugated (bs-1495R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Mouse Kidney lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Hela cell lysates probed with Occludin Polyclonal Antibody, Unconjugated (bs-1495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1495R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1495R-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

U251 lysates probed with Rabbit Anti-DRD2 Polyclonal Antibody, Unconjugated (bs-1008R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DRD2 Polyclonal Antibody Polyclonal Antibody, Unconjugated (bs-1008R) at 1:400 overnight at 4\u00b0C, followed by an SP Kit (Rabbit) and DAB staining.
Lane 1: Mouse BV-2 cell lysates; Lane 2: Rat RSC96 cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human U-87MG cell lysates probed with DRD2 Polyclonal Antibody, Unconjugated (bs-1008R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1008R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1008R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1008R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat skin labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse kidney cells labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0766R) 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded Lymphocytes labeled with (bs-0766R) Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated followed by incubation with a conjugated secondary antibody.
Formalin-fixed and paraffin embedded Lymphocytes labeled with Rabbit Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) followed by incubation with a conjugated secondary antibody and DAPI staining.

Catalog #1: bs-0766R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3584R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-0766R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0766R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-CD4 Polyclonal Antibody, Unconjugated (bs-0647R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat lung labeled with Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0647R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Image was kindly submitted by Dr.David M Burmeister from US Army Institute of Surgical Research. Pig lymph nodes stained with Rabbit Anti-CD4 Polyclonal Antibody(bs-bs-0647R)at 1:300 for one hour at room temperature.\\n
Mouse spleen cells probed with CD4 Polyclonal Antibody, PE Conjugated (bs-0647R-PE; purple) for 30 minutes at 1:50.

Catalog #1: bs-0647R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0647R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-0647R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: HRP

Catalog #1: bs-2790R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-2790R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-2790R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD134 Polyclonal Antibody, Unconjugated (bs-2685R) followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2685R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2685R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2685R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2081R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2081R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-2081R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TTR Prealbumin beta Polyclonal Antibody, Unconjugated (bs-0152R) at 1:400 overnight at 4\u00b0C, DAB staining.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Prealbumin Antibody(bs-0152R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TTR\/Prealbumin) polyclonal Antibody, Unconjugated (bs-0152R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0152R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0152R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0152R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated (bs-0756R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image provided by One World Lab validation program. U138 cells were stained with bs-0756R Rabbit Anti-Vimentin Polyclonal Antibody at 1:100 dilution.

Catalog #1: bs-0756R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0756R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0756R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0080R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CD20 Polyclonal Antibody (bs-0080R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human larynocarcinoma labeled with Anti-CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Bone lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-0080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0080r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-0080R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-1970R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
RSC96 probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1970R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1970R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

293T cell lysates probed with LAG-3 Polyclonal Antibody, Unconjugated (bs-2646R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
K562 cell lysates probed with LAG-3 Polyclonal Antibody, Unconjugated (bs-2646R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Ctll-2 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lymphocyte Activation Gene 3Antibody(bs-2646R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-2646R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2646R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-2646R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

L1 human colon carcinoma lysates L2 rat brain lysates probed with Anti GPR15 Polyclonal Antibody, Unconjugated (bs-3699R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD. Observed band size:40kD.\\n
Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3699R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3699R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3699R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebral cortex; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NeuN Polyclonal Antibody, Unconjugated bs-1613R at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FOX3\/NeuN Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1613R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1613R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1613R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Integrin beta 1\/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human A431 cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Rat Ear lysates; Lane 4: Mouse Ear lysates; Lane 5: Mouse Urinary bladder lysates probed with Integrin beta 1 Polyclonal Antibody, Unconjugated (bs-0486R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Integrin beta 1) polyclonal Antibody, Unconjugated (bs-0486R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-0486R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-0486R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Biotin

Catalog #1: bs-0486R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: HRP