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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-17394R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-20154R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-20249R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-17394R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-14567R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-17300R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-14567R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-14567R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-17300R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-20595R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-17300R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-0023R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0023R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-20595R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Mouse Placenta lysates probed with GSK3B Polyclonal Antibody, Unconjugated bs-0023R at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Muscle lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-0023R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0023R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (BSA or normal goat serum) at 37\u00b0C for 20min; Antibody incubation with TLR4 Polyclonal Antibody, Unconjugated bs-20595R at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Mouse Liver lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Jurkat cells probed with TLR4 Polyclonal Antibody, unconjugated (bs-20595R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Lane 1: Human H9C2 cell lysates; Lane 2: Human K562 cell lysates probed with TLR4 Polyclonal Antibody, Unconjugated (bs-20595R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-20595R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
RSC96 probed with alpha Tubulin (4G1) Monoclonal Antibody, Unconjugated (bsm-50179M) at 1:100 for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with alpha Tubulin (4G1) monoclonal Antibody, Unconjugated (bsm-50179M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Lane 1: Hela; Lane 2: 293T; Lane 3: MDA-MB-231; Lane 4: A549; Lane 5: A431; Lane 6: RSC96; Lane 7: VSC4.1; Lane 8: ASPC-1; Lane 9: B16; Lane 10: BV2; Lane 11: H9C2; Lane 12: Hep2G;Lane 13: K562; Lane 14: U937; probed with alpha Tubulin (4G1) Monoclonal Antibody, unconjugated (bsm-50179M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.\r\n

Catalog #1: bsm-50179M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, All

Conjugate: Unconjugated

Catalog #1: bs-12999R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-24134R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-24134R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-24134R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bsm-33050m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33050m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: Hela KO EGFR cell lysates; Lane 2: Hela cell lysates probed with EGFR Monoclonal Antibody, Unconjugated (bsm-33050M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (bsm-33050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with EGFR monoclonal antibody, unconjugated (bsm-33050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33050m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-50553R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: Phospho Mu Opioid Receptor (blue line), 0.2ug\/100ul; Mu Opioid Receptor(red line), 0.2ug\/100ul\\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n
Image kindly provided by Dr. Stefen Clemens of East Carolina University. pMOR protein expression levels in the spinal cords of WT and D3KO mice (top lanes), and their respective \u00df-actin protein expression (bottom panels). Rabbit Anti-mu Opioid Receptor (Ser375) Polyclonal Antibody was used at a dilution of 1:1000.

Catalog #1: bs-3724R

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Lane 1: L6; Lane 2: NIH\/3T3; Lane 3: T47D; Lane 4: A2058; Lane 5: HepG2; Lane 6: K562; Lane 7: HeLa Cell lysates; probed with Hsp90 (3C9) Monoclonal Antibody, unconjugated (bsm-51215M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human U251 cell lysates ; Lane 7: Human Molt-4 cell lysates ; Lane 8: Human HL60 cell lysates ; Lane 9: Human A549 cell lysates probed with HSP90 Monoclonal Antibody, Unconjugated (bsm-51215M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51215m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPT1 (10G4) Monoclonal Antibody (bsm-51262M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells probed with using PPT1 (10G4) Monoclonal Antibody (green) unconjugated bsm-51262M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51262M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FYN (2C7) Monoclonal Antibody (bsm-51263M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
293 cells probed with FYN (2C7) Monoclonal Antibody, unconjugated (bsm-51263M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Human Hela Cells probed with FYN (2C7) Monoclonal Antibody, Unconjugated (bsm-51263M) (green) at 1:25 for 60 minutes followed by a Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400. The graph shows FYN staining compared a mouse IgG1 isotype control (blue).

Catalog #1: bsm-51263M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; Lane 2: U266 Cell lysates; probed with CDKN1B (10B7) Monoclonal Antibody, unconjugated (bsm-51281M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDKN1B (10B7) Monoclonal Antibody (bsm-51281M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51281M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HT-29 Cell lysates; probed with AURKA (4C5) Monoclonal Antibody, unconjugated (bsm-51279M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with AURKA (4C5) Monoclonal Antibody (bsm-51279M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51279M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: VWF protein; probed with VWF (907CT12.1.9) Monoclonal Antibody, unconjugated (bsm-51280M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VWF (907CT12.1.9) Monoclonal Antibody (bsm-51280M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51280M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: NIH\/3T3; Lane 3: C6 cell lysate probed with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51282M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: THP-1 Cell lysates; Lane 2: mouse liver lysates; Lane 3: mouse testis lysates; probed with ATG3 (7C2) Monoclonal Antibody, unconjugated (bsm-51284M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51285M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51284M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: C2C12 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; Lane 5: C6 Cell lysates; probed with RPTOR (14C4) Monoclonal Antibody, unconjugated (bsm-51285M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RPTOR (14C4) Monoclonal Antibody (bsm-51285M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A549 cell lysates; Lane 2: K562 cell lysates probed with Raptor\/RPTOR (14C4) Monoclonal Antibody, Unconjugated (bsm-51285M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51285M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with ATG4A (1458CT808.66.25.69) Monoclonal Antibody (bsm-51292M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG4A (1458CT808.66.25.69) Monoclonal Antibody (bsm-51292M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
K562 lysates probed with ATG4A (14C9) Monoclonal Antibody, Unconjugated (bsm-51292M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bsm-51292M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: HeLa Cell lysates; probed with FGFR1 (4C3) Monoclonal Antibody, unconjugated (bsm-51288M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FGFR1 (4C3) Monoclonal Antibody (bsm-51288M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells; antibody incubation with FGFR1 (4C3) Monoclonal Antibody, unconjugated (bsm-51288M) at 1:25, followed by a conjugated secondary and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FGFR1) Monoclonal Antibody, Unconjugated (bsm-51288M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51288M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
Lane 1: Jurkat Cell lysates; Lane 2: MOLT-4 Cell lysates; Lane 3: mouse thymus Cell lysates; probed with Zap70 (4C2) Monoclonal Antibody, unconjugated (bsm-51298M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using Zap70 (4C2) Monoclonal Antibody, bsm-51298M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51298M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 cell lysates; probed with DDR1 (4C4) Monoclonal Antibody, unconjugated (bsm-51295M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with DDR1 (4C4) Monoclonal Antibody (bsm-51295M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51295M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51296M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: U-251MG Cell lysates; Lane 2: A549 Cell lysates; probed with FES (7C7) Monoclonal Antibody, unconjugated (bsm-51297M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: U87-MG Cell lysates; probed with FES (7C7) Monoclonal Antibody, unconjugated (bsm-51297M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FES (7C7) Monoclonal Antibody (bsm-51297M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51297M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (LAMP1) monoclonal Antibody, unconjugated (bsm-51301M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51301M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HT-1080 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-51030M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with LAMP1 monoclonal antibody, unconjugated (bsm-51301M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-51301m

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells labeled with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Jurkat cells probed with using HINT1 (5C3) Monoclonal Antibody bsm-51304M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared (green) compared to an isotype control of mouse IgG1 (blue).
Lane 1: Jurkat; Lane 2: Raji; Lane 3: HepG2; Lane 4: Rat Brain lysates; Lane 5: Mouse Liver lysates; probed with HINT1 (5C3) Polyclonal Antibody, unconjugated (bsm-51304M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51304M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CSF1R (6C7) Monoclonal Antibody (bsm-51303M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining
Human placenta lysates probed with CSF1R (6C7) Monoclonal Antibody, unconjugated (bsm-51303M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CSF1R (6C7) Monoclonal Antibody (bsm-51303M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
U-87MG cells probed with using CSF1R (6C7) Monoclonal Antibody bsm-51303M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG1 (blue).

Catalog #1: bsm-51303M

Applications: WB, ELISA, FCM, IHC-P

Reactivity:

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with IRF3 (2C4) Monoclonal Antibody (bsm-51312M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cells probed with using IRF3 (2C4) Monoclonal Antibody bsm-51312M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG (blue).
Lane 1: COS-7; Lane 2: Daudi; Lane 3: HepG2; Lane 4: HT-29; Lane 5: Jurkat; Lane 6: MCF-7; probed with IRF3 (2C4) Polyclonal Antibody, unconjugated (bsm-51312M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antibody incubation with IRF3 (2C4) Monoclonal Antibody, Unconjugated (bsm-51312M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51312M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity:

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeled with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1\/25 dilution, followed by secondary antibody (green) and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with KRT19 (979CT14.3.1) Monoclonal Antibody (bsm-51308M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51308M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cell lysates; Lane 2: A431 Cell lysates; Lane 3: THP-1 Cell lysates; Lane 4: 293T\/17 Cell lysates; probed with BID (9C7) Monoclonal Antibody, unconjugated (bsm-51306M) at 1:x000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 cells labeled with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded human bladder tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BID (9C7) Monoclonal Antibody (bsm-51306M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51306M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labeled with LCK (6C6) Monoclonal Antibody (bsm-51313M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Jurkat cell lysates probed with LCK (6C6) Polyclonal Antibody, unconjugated (bsm-51313M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using LCK (6C6) Monoclonal Antibody bsm-51313M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody (green) at 1:400 compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51313M

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated