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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-33219M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy3

Catalog #1: bsm-33219M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy5

Catalog #1: bsm-33219M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Cy7

Catalog #1: bsm-33219M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: FITC

Catalog #1: bsm-33293M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

293T lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
A549 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 lysates probed with GSK3B (3A6) Monoclonal Antibody, unconjugated (bsm-33293M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSK3B (3A6) Monoclonal Antibody, Unconjugated (bsm-33293M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bsm-33293M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bsm-33293M-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bsm-33293M-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bsm-33293M-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bsm-33293M-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Tissue\/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal mouse serum, C-0082) at 37\u00b0C for 20 min; Incubation: Anti-Cyclin B1 Monoclonal Antibody, conjugated (bsm-33042M-AF350) 1:100, 2 hours at 37\u00b0C; PI (red, S0009) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Histone H3\/HIST3H3 (3G1)) Monoclonal Antibody, Unconjugated (bsm-33042M ) at 1:500 overnight at 4\u00b0C, followed by a conjugated Goat Anti- Mouse IgG antibody (YF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33042m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33042m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with Histone H3\/HIST3H3\/HRP monoclonal antibody, conjugated (bsm-33042M-HRP) at 1:50000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Histone H3 Monoclonal Antibody (bsm-33042M-HRP) at 1:200 overnight at 4\u00b0C, and DAB (C-0010) staining.

Catalog #1: bsm-33042m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33042m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33042m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33042m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33042m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33187m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9)) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACTA2 (3F9)) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33187m-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-FITC) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33187m-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: alpha smooth muscle Actin Monoclonal Antibody, conjugated (bsm-33187M-Cy5) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33187m-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035M-HRP) at 1:1000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.

Catalog #1: bsm-33035m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCNA (3G1) Monoclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-33035m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33035m-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33035m-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035m-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33035m-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33170m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin\/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin\/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin\/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Vimentin\/Biotin Monoclonal Antibody, Unconjugated(bsm-33170M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33170m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Lane 1: HeLa (30ug); Lane 2: HeLa KO Vimentin lysate (30ug) probed with Vimentin (3B7) Monoclonal Antibody, unconjugated (bsm-33170M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Vimentin(3B7) ) Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Vimentin(3B7) ) Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Vimentin(3B7) ) Monoclonal Antibody, Unconjugated (bsm-33170M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33170m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33004M-Biotin

Applications: WB

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33216M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33216M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33117m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33117m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

HepG2 cell lysate probed with MEK2 (4C3) Monoclonal Antibody (bsm-33216M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 Monoclonal Antibody (bsm-33216M) at 1:400 overnight at 4\u00b0C, followed by SP Kit(Mouse) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK2 (4C3) Monoclonal Antibody, Unconjugated (bsm-33216M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33216M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: Rela-KO cell lysates; Lane 2: Hela cell lysates probed with NFKB p65 (7G6) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with NFKB p65 monoclonal antibody, unconjugated (bsm-33117M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, unconjugated (bsm-33117M) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-40296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated(bsm-33117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33117m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate; Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate probed with His tag (6E6) Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with His tag Monoclonal Antibody, Unconjugated (bsm-33004M) at 1:200000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33004M

Applications: WB

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bsm-33033m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Biotin

Catalog #1: bsm-33004M-HRP

Applications: WB

Reactivity: Species independent

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33033m

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bsm-33033m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: HRP

Catalog #1: bsm-33040m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33040m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP