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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-51223M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51218M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HeLa; Lane 2: HepG2; Lane 3: HIN\/3T3 cell lysate probed with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Catalog #1: bsm-51220M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: SK-BR-3 Cell lysates; Lane 5: T47D Cell lysates; Lane 6: MDA-MB-453 Cell lysates; Lane 7: mouse testis lysates; probed with ACE2 (881CT16.4.4) Monoclonal Antibody, unconjugated (bsm-51221M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ACE2(1C4) ) Monoclonal Antibody, Unconjugated (bsm-51221M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51221M

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51229M

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51226M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51227M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51228M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51236M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PIN1 monoclonal antibody, unconjugated (bsm-51230M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51230m-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51231M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51233M

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: MCF-7 Cell lysates; probed with MGMT (888CT22.2.4) Monoclonal Antibody, unconjugated (bsm-51234M) at 1:25 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human small intestine tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MGMT (888CT22.2.4) Monoclonal Antibody (bsm-51234M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51234M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51235M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51237M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (bsm-51239M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51239m-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: CEM Cell lysates; Lane 2: 293; Lane 3: C6 probed with bsm-51241M SUMO2 (7A3) Monoclonal Antibody at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HeLa cells stained with SUMO2 (7A3) Monoclonal Antibody (bsm-51241M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), cytoplasmic actin (red).

Catalog #1: bsm-51241M

Applications: WB, IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51242M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Adipose tissue lysates; Lane 2: Mouse Breast tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Thymus tissue lysates probed with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Fat; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51247m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51243M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51244M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VEGFR3 (818CT12.1.1) Monoclonal Antibody (bsm-51245M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with VEGFR3 (818CT12.1.1) Monoclonal Antibody (bsm-51245M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51245M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51250M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51251M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51252M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51259M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51255M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51254M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51256M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51258M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51269M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

KLF4 recombinant protein (20ug) probed with bsm-51264M KLF4 (5A1) Monoclonal Antibody at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: KLF4 protein; probed with KLF4 (5A1) Monoclonal Antibody, unconjugated (bsm-51264M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51264M

Applications: WB, IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-51266M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with EPHA5 (46CT61.6.4) Monoclonal Antibody (bsm-51268M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51268M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: A431 probed with bsm-51265M PINK1 (38CT20.8.5) Monoclonal Antibody at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51265m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with SOD2 (37CT127.5.11.6) Monoclonal Antibody (bsm-51267M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Siha cell lysates; Lane 2: HepG2 cell lysates; Lane 3: K562 cell lysates; Lane 4: U251 cell lysates probed with SOD2 (6C11) Monoclonal Antibody, Unconjugated (bsm-51267M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51267M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51324m

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51271M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51323M

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51393M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Mouse brain lysates; Lane 2: HeLa Cell lysates; Lane 3: Y79 Cell lysates; Lane 4: CEM Cell lysates; Lane 5: PC-12 Cell lysates; probed with TBB5 (7D3) Monoclonal Antibody, unconjugated (bsm-51387M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A549 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), cytoplasmic actin (red).

Catalog #1: bsm-51387M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Sample Tissue\/Cell lysates; Lane 2: Sample Tissue\/Cell lysates; Lane 3: Sample Tissue\/Cell lysates; probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Jurkat Cell lysates; Lane 2: MCF-7 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; probed with RPS6KB2 (164CT21.2.2) Monoclonal Antibody, unconjugated (bsm-51388M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51388M

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human Liver Lysates; Lane 2: MCF-7 Cell Lysates; Lane 3: HepG2 Cell Lysates; Lane 4: HeLa Cell Lysates; Lane 5: PC-12 Cell Lysates; probed with PSMA5 (426CT8.5.1) Monoclonal Antibody, unconjugated (bsm-51389M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PSMA5 (426CT8.5.1) Monoclonal Antibody (bsm-51389M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51389M

Applications: WB, IHC-P, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51394M

Applications: WB

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: Rat Liver lysates; Lane 3: MCF-7 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; probed with PHB (12C6) Monoclonal Antibody, unconjugated (bsm-51395M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PHB (12C6) Monoclonal Antibody (bsm-51395M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51395M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: PC-12 probed with TOP1 (1C6) Monoclonal Antibody, unconjugated (bsm-51396M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Hela cells stained with TOP1 (1C6) Monoclonal Antibody, bsm-51396M at 1:25 dilution, followed by Alexa Fluor\u00ae 488 goat anti-mouse lgG (green), compared to mouse IgG1 isotype control (blue).
Lane 1: Molt-4 cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Siha cell lysates; Lane 4: K562 cell lysates probed with TOP1 (1C6) Monoclonal Antibody, Unconjugated (bsm-51396M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51396M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HepG2 Cell lysates; Lane 3: NIH\/3T3 Cell lysates; Lane 4: mouse brain lysates; Lane 5: C6 Cell lysates; Lane 6: PC-12 Cell lysates; probed with YWHAZ (1B6) Monoclonal Antibody, unconjugated (bsm-51397M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with YWHAZ (1B6) Monoclonal Antibody, Unconjugated (bsm-51397M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-51397M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Daudi Cell lysates; Lane 2: Raji Cell lysates; probed with BCL10 (5C2) Monoclonal Antibody, unconjugated (bsm-51398M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with BCL10 (5C2) Monoclonal Antibody (bsm-51398M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Hela cells probed with using BCL10 (5C2) Monoclonal Antibody (green) unconjugated bsm-51398M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared to an isotype control of mouse IgG (blue).
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with BCL10 (5C2) Monoclonal Antibody, Unconjugated (bsm-51398M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51398M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated