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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-ELK1 Polyclonal Antibody, Unconjugated (bs-1398R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain lysates probed with ELK1 Polyclonal Antibody, Unconjugated (bs-1398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Blank control:Hela. \r\nPrimary Antibody (green line): Rabbit Anti-ELK1 antibody (bs-1398R) \r\nDilution: 1ug\/Test; \r\nProtocol\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

Catalog #1: bs-1398R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SRF\/SAP2\/MCM1 Polyclonal Antibody, Unconjugated (bs-1401R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse large intestine lysates probed with Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Mouse muscle lysates probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Mouse NIH\/3T3 cells probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-1401R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-SREBP-1 Polyclonal Antibody, Unconjugated (bs-1402R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti SREBP-1 Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti SREBP-1 Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse liver lysates; Lane 2:Rat Liver lysates probed with SREBP1 Polyclonal Antibody, Unconjugated (bs-1402R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1402R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-HNF1\/TCF1\/HNF1A Polyclonal Antibody, Unconjugated (bs-1405R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (HNF1A) polyclonal Antibody, Unconjugated (bs-1405R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1405R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1406R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1407R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical carcinoma Rabbit Anti-HSTF2\/HSF2 Polyclonal Antibody, Unconjugated (bs-1409R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-HSTF2\/HSF2 Polyclonal Antibody, Unconjugated (bs-1409R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-HSTF2\/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-1409R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1408R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1410R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-1411R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded transplanted tumor of cervical carcinoma in nude mice labeled with Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse cervical cancer labeled with Anti-MAT-1 Polyclonal Antibody, Unconjugated (bs-1412R) followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with MTA1 Polyclonal Antibody, Unconjugated (bs-1412R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1412R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1414R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, GuineaPig

Conjugate: Unconjugated

Catalog #1: bs-1415R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-NFATC1\/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Hippocampal tissue labeled with Anti-NFATc1\/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) followed by conjugation to the secondary antibody
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:400 overnight at 4\u00b0C, DAB staining.
Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFAT2 Polyclonal Antibody(bs-1417R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1417R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1418R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Lane 1:mouse kidney lysates Lane 2: mouse brain lysates probed with Anti-MC-1R\/MC1 Receptor Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 35kD. Observed band size: 35kD.
paraformaldehyde-fixed, paraffin embedded human melanoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-MC-1R Polyclonal Antibody, Unconjugated (bs-1419R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human 293T Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min. Cells were stained with MC-1R\/MC1 Polyclonal Antibody, Alexa Fluor 488 Conjugated (bs-1419R-A488) at 1:100 for 30 min on ice. Primary Antibody staining (green) is compared to unstained cells (dark blue), and isotype control (orange).
Lane 1: MOLT-4 Cells; Probed with MC-1R\/MC1 Polyclonal Antibody, Unconjugated (bs-1419R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-1419R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1420R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1422R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-1421R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-EPOR Polyclonal Antibody (bs-1424R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled withAnti-EPOR Polyclonal Antibody (bs-1424R), Unconjugated at 1:100 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Human K652 Cell lysate (30ug), probed with EPOR Polyclonal Antibody, Unconjugated (bs-1424R) at 1:1000 overnight at 4˚C. Followed by incubation with secondary antibody at 1:20000 for 90min at 37C.
Human K652 Cell lysate (30ug), probed with EPOR Polyclonal Antibody, Unconjugated (bs-1424R) at 1:300 overnight at 4˚C. Followed by incubation with secondary antibody at 1:20000 for 90min at 37C.

Catalog #1: bs-1424R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

HeLa lysates probed with Rabbit Anti-Claudin 1 Polyclonal Antibody (bs-1428R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Claudin 1 Polyclonal Antibody, Unconjugated (bs-1428R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human HepG2 cell lysates probed with Claudin 1 Polyclonal Antibody, Unconjugated (bs-1428R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1428R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1429R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

This image was generously provided by Piotr Mamczur at University of Wroclaw as part of the Bioss Discovery Program. Mouse squamous cell carcinoma (KLN-205 cell line) were probed with Rabbit Anti-RPS6KB1 Polyclonal Antibody (bs-1426R) at 1:100 followed by at FITC conjugated secondary antibody and DAPI staining
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RPS6KB1 Polyclonal Antibody, Unconjugated (bs-1426R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1426R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1432r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Images provided the Independent Validation Program (badge number 029646)Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-MEK1 Polyclonal Antibody (bs-1433R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody. \\n
Image provided by the Independent Validation Program (badge number 29760). Lane 1: HeLa cell extract, Lane 2: NIH\/3T3 cell extracts, Lane 3: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MEK1 Polyclonal Antibody, Unconjugated (bs-1433R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.

Catalog #1: bs-1433R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-IGFBP3 Polyclonal Antibody, Unconjugated(bs-1434R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG,Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: HepG2 cell lysates probed with IGFBP3 Polyclonal Antibody, Unconjugated (bs-1434R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1434R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1437R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1438R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-1440R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) followed by conjugation to the secondary antibody and DAB staining
Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody (bs-1441R) at 1:50 for two hours at room temperature (green channel).
BV2 (Human) lysates probed with CXCL16 Polyclonal Antibody, Unconjugated (bs-1441R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1441R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1442R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-VCP Polyclonal Antibody, Unconjugated (bs-1443R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VCP Polyclonal Antibody, Unconjugated (bs-1443R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1443R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1445R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1446R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat pancreas labeled with Anti-Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse heart lysates probed with Prohibitin Polyclonal Antibody, Unconjugated (bs-1449R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-1449R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse eye labeled with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse lung lysates Lane 2: mouse muscle lysates probed with Anti Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 20kD. Observed band size: 20kD.
RSC96 cells probed with Caveolin-1 Polyclonal Antibody, Unconjugated (bs-1453R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-1453R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ErbB-3\/HER3 Polyclonal Antibody, Unconjugated (bs-1454R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-ErbB-3\/HER3 Polyclonal Antibody, Unconjugated (bs-1454R) followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1454R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:rat heart lysates Lane 2: rat brain lysates probed with Anti A2AR\/Adenosine A2a receptor Polyclonal Antibody, Unconjugated (bs-1456R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 64kD. Observed band size: 64kD.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-A2AR\/Adenosine A2a Polyclonal Antibody, Unconjugated (bs-3927R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-1456R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Rabbit Anti-CD118 Polyclonal Antibody (bs-1458R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
MCF-7 lysates probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Catalog #1: bs-1458R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-IL-6R Beta\/CD130\/gp130 Polyclonal Antibody, Unconjugated (bs-1459R) followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with IL-6R Beta\/CD130\/gp130 Antibody, Unconjugated (bs-1459R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Human Raji cells probed with IL-6R beta Polyclonal Antibody, Unconjugated (bs-1459R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Catalog #1: bs-1459R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: HL60 cell lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Lymph node lysates; Lane 2: Rat Lymph node lysates; Lane 3: Rat Thymus lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human K562 cell lysates; Lane 2: Human Jurkat cell lysates probed with IL-23R Polyclonal Antibody, Unconjugated (bs-1460R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

Catalog #1: bs-1460r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1461R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Rabbit Anti-GnRHR Polyclonal Antibody, Unconjugated(bs-1464R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
U251 lysates probed with GnRHR Polyclonal Antibody, unconjugated (bs-1464R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Jurkat cell lysates probed with GnRHR Polyclonal Antibody, Unconjugated (bs-1464R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1464R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1466R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates probed with GRP75 Polyclonal Antibody, Unconjugated (bs-1469R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1469R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1470R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFI16\/MNDA Polyclonal Antibody, Unconjugated (bs-1472R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IFI16\/MNDA Polyclonal Antibody, Unconjugated (bs-1472R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1472R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1473R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated