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Primary Antibodies

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Catalog #1: bs-4587R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-4587R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-4587R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-4587R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: Cy5

Catalog #1: bs-4587R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: Cy3

Catalog #1: bs-4587R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: FITC

Catalog #1: bs-4587R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: Cy7

Catalog #1: bs-4587R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-4587R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine, Pig, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-4834R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-4834R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Unconjugated

Catalog #1: bs-4834R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-4834R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Biotin

Catalog #1: bs-4834R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: HRP

Catalog #1: bs-4834R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-4834R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-4834R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy5

Catalog #1: bs-4834R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy3

Catalog #1: bs-4834R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy7

Catalog #1: bs-4834R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: FITC

Catalog #1: bs-4834R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-4834R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: PE

Catalog #1: bs-4834R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: ALEXA FLUOR® 750

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Hsc70 Polyclonal Antibody(bs-5117R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Hsc70) polyclonal Antibody, Unconjugated (bs-5117R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5117R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Unconjugated

Catalog #1: bs-20379R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Bovine

Conjugate: Unconjugated

Catalog #1: bs-20379R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Bovine

Conjugate: Biotin

Catalog #1: bs-20379R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Bovine

Conjugate: HRP

NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-RPS6KB1 (Ser417) Polyclonal Antibody(bs-5668R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-RPS6KB1 (Ser417)) polyclonal Antibody, Unconjugated (bs-5668R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5668R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Unconjugated

Catalog #1: bs-5668R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-5668R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Biotin

Catalog #1: bs-5668R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: HRP

Catalog #1: bs-5668R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-5668R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Cy3

Catalog #1: bs-5668R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-5668R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-5668R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-5668R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Cy5

Catalog #1: bs-5668R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: Cy7

Catalog #1: bs-5668R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-5668R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-5668R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Others

Conjugate: FITC

Catalog #1: bs-5703R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: Unconjugated

Catalog #1: bs-5703R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Bovine

Conjugate: Biotin

Catalog #1: bs-5703R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-5703R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-5703R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-5703R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-5703R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: Cy3