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Primary Antibodies

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Catalog #1: bs-5703R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: Cy7

Catalog #1: bs-5703R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-5703R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: Cy5

Catalog #1: bs-5703R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: FITC

Catalog #1: bs-5703R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-5911R

Applications: WB, ELISA, IF(IHC-P)

Reactivity: Human, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-6319R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 350

Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Raw264.7 Cells (30ug); Lane 2: Mouse Liver (40ug); Probed with IL-1 Beta Polyclonal Antibody, unconjugated (bs-6319R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Raw264.7 cell lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-6319R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IL-1 Beta Polyclonal Antibody(bs-6319R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-6319R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-6319R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Biotin

Catalog #1: bs-6319R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: HRP

Catalog #1: bs-6319R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-6319R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-6319R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy3

Catalog #1: bs-6319R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-6319R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-6319R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy5

Catalog #1: bs-6319R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: Cy7

Catalog #1: bs-6319R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: FITC

Catalog #1: bs-6319R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-6319R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-6370R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-6370R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Others

Conjugate: Biotin

Catalog #1: bs-6370R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: Unconjugated

Catalog #1: bs-6370R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Others

Conjugate: HRP

Catalog #1: bs-6370R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-6370R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-6370R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-6370R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-6370R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: Cy3

Catalog #1: bs-6370R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: Cy5

Catalog #1: bs-6370R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-6370R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: Cy7

Catalog #1: bs-6370R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Bovine, Others

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-6425R-A488

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 488

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4\u00b0C, DAB staining.
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (TRP12\/TRPV4) polyclonal Antibody, Unconjugated (bs-6425R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-6425R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Bovine

Conjugate: Unconjugated

Catalog #1: bs-6425R-A350

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-6425R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Bovine

Conjugate: Biotin

Catalog #1: bs-6425R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Bovine

Conjugate: HRP

Catalog #1: bs-6425R-Cy5

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: Cy5

Catalog #1: bs-6425R-Cy3

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: Cy3

Catalog #1: bs-6425R-A647

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-6425R-A594

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-6425R-A555

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-6425R-FITC

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: FITC

Catalog #1: bs-6425R-A680

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-6425R-Cy7

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: Cy7

Catalog #1: bs-6425R-A750

Applications: WB, FCM

Reactivity: Human, Bovine

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-0465R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Others, Zebrafish

Conjugate: ALEXA FLUOR® 680